肾去交感支配对心肌梗死后大鼠心肌纤维化和转化生长因子-β1的影响  

Effect of renal sympathetic denervation on myocardial fibrosis and the expression of TGF-β1 in rats with myocardial infarction

作　　者：郑晓新[1] 侯果[1] 邱璇[1] 蒋学俊[1] ZHENG Xiao-xin HOU Guo QIU Xuan et al.(Department of Cardiology, Renmin Hospital of Wuhan University, Hubei Key Laboratory of Cardiology, Cardiovascular Research Institute, Wuhan 430060, Chin)

机构地区：[1]武汉大学人民医院心血管内科心血管病湖北省重点实验室武汉大学心血管病研究所,湖北省武汉市430060

出　　处：《中国心血管病研究》2016年第9期845-849,863,864,共7页Chinese Journal of Cardiovascular Research

基　　金：中央高校专项科研项目基金（项目编号：2042015kf0074）

摘　　要：目的 探讨肾去交感神经术（RSD）对心肌梗死大鼠心肌纤维化和转化生长因子-β1（TGF-β1）表达水平的影响.方法 52只雄性SD大鼠随机分为4组：正常对照组（Control组）、肾去交感组（RSD组）、心肌梗死组（MI7d＋Sham组）和肾去交感干预组（MI7d＋RSD组）.MI7d＋Sham组和MI7d＋RSD组分别给予前降支冠脉结扎术制备心梗模型,7d后行肾交感假手术或去交感术.心脏彩超评估大鼠左心室收缩末期直径（LVESD）、左心室射血分数（LVEF）和左心室短轴缩短率（LVFS）.Masson法检测大鼠左心室心肌胶原容积分数（CVF）.RT-PCR法和Western-blot法分别检测大鼠左室心肌TGF-β1 mRNA和TGF-β1蛋白的相对表达水平.结果 与MI7d＋Sham组相比,M17d＋RSD组大鼠LVESD明显减小[LVESD （3.13±0.25）mm比（5.23±0.15）mm,P＜0.01],而LVFS和LVEF均增高[LVFS（38.2±4.5）％比（23.1±3.3）％,P＜0.01;LVEF（61.7±1.3）％比（40.4±2.7）％,P＜0.01].与MI7d＋Sham组相比,Mt7d＋RSD组胶原容积分数减少[（42.66±7.55）％比（59.33 ±9.79）％,P＜0.01],TGF-β1 mRNA（1.47±0.09比2.00±0.09,P＜0.01）和TGF-βI蛋白（0.46±0.04比0.73±0.02,P＜0.01）的相对表达量均减少.结论 肾去交感神经术能抑制心肌梗死大鼠心肌纤维化并改善心功能,可能与下调TGF-β1的表达有关.Objective To observe the effect of renal sympathetic denervation （RSD） on myocardial fibro- sis and the expression of transforming growth factor-β1 （TGF-β1 ） in myocardial infarction （MI） rats. Methods 52 SPF level SD rats were randomly divided into four groups, control group, RSD group, MI7d＋Sham group and MITd＋RSD group. Bilateral renal sympathectomy or sham operation were performed in RSD group and MI7d＋RSD group or MI7d＋Sham group, separately. Ultrasound was adopted to evaluate the changes of left ventricular end- systolic diameter （LVESD）, left ventricular fractional shortening （LVFS） and left ventricular ejection fraction （LVEF）. Masson staining was used to measure the collagen volume fraction （CVF）. RT-PCR and western-blot methods for the detection of myocardial mRNA and protein expression levels of TGF-β1, respectively. Results LVESD was lower in MI7d ＋RSD group than those in MI7d ＋Sham group, and LVFS and LVEF were higher [LVESD （3.13±0.25）mm vs （5.23±0.15）mm, P〈0.01, LVFS （38.2±4.5）% vs （23.1±3.3）%, P〈0.01, LVEF （61.7±1.3）% vs （40.4±2.7）%, P〈0.01 ]. Compared with MI7d＋Sham group,CVF was lower in MI7d＋RSD group [（42.66±7.55）% vs （59.33±9.79）%, P〈0.01], and the relative expression of TGF-β1 mRNA was lower than that in MI7d＋Sham group（ 1.47±0.09 vs 2.00±0.09, P〈0.01 ）, and the relative expression of TGF-β1 protein was lower than that in MI group （0.46±0.04 vs 0.73±0.02, P〈0.01 ）. Conclusion Renal sympathetic denervation can inhibit myocardial fibrosis in rats post-MI and improve heart function, which may be achieved by inhibiting the expression of myocardial TGF-β1.

关 键 词：肾去交感支配 心肌梗死 心肌纤维化 转化生长因子-Β1 

分 类 号：Q95-33[生物学—动物学] R542.22[医药卫生—心血管疾病]