Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean  

作　　者：CHENG Wen GAO Jin-shan FENG Xing-xing SHAO Qun YANG Su-xin FENG Xian-zhong 

机构地区：[1]Key Laboratory of Systems Biology in Universities of Shandong/College of Life Science, Shandong Normal University [2]Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences

出　　处：《Journal of Integrative Agriculture》2016年第10期2228-2236,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31171571 and 31571692);the One Hundred Person Project of the Chinese Academy of Sciences

摘　　要：Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M-2 population. Two of these mutants, Gmdwf1（Glycine max dwarf 1） and Gmdwf2（Glycine max dwarf 2）, were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin（GA） and brassinolide（BR） treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M-2 population. Two of these mutants, Gmdwf1（Glycine max dwarf 1） and Gmdwf2（Glycine max dwarf 2）, were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin（GA） and brassinolide（BR） treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.

关 键 词：soybean dwarf mutant mapping BR GA 

分 类 号：S565.1[农业科学—作物学]