链霉菌Streptomyces sp. CPCC 200497次级代谢产物bohemamines的分离和鉴定  

Isolation and identification of bohemamines as secondary metabolites from Streptomyces sp. CPCC 200497

作　　者：江冰娅[1] 赵薇[1] 李书芬[1] 刘红宇[1] 余利岩[1] 武临专[1] JIANG Bing-ya ZHAO Wei LI Shu-fen LIU Hong-yu YU Li-yan WU Lin-zhuan(Key Laboratory of Biotechnology of Antibiotics of National Health and Family Planning Commission, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100050, China)

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所卫计委抗生素生物工程重点实验室,北京100050

出　　处：《中国医药生物技术》2016年第5期394-399,共6页Chinese Medicinal Biotechnology

基　　金：国家自然科学基金(81302676);国家微生物资源基础平台(NIMR-2015-3)

摘　　要：目的对醌霉素产生菌——Streptomyces sp.CPCC 200497产生的次级代谢产物进行深入研究。方法将Streptomyces sp.CPCC 200497进行固体发酵,用高效硅胶板薄层色谱、中低压制备色谱和高效液相色谱等方法对发酵培养物的乙酸乙酯提取物进行分析和纯化;通过质谱和NMR等对获得的单体化合物进行结构鉴定。结果从Streptomyces sp.CPCC 200497发酵物的乙酸乙酯提取物中发现了两个吡咯里西啶类生物碱,分别为bohemamine和bohemamine B。结论从醌霉素产生菌Streptomyces sp.CPCC 200497中首次分离得到bohemamine类化合物,并通过1D和2D-NMR对化合物的核磁数据进行了准确归属,为国内首次对该类化合物的分离鉴定进行报道。Objective To study in depth the secondary metabolites from Streptomyces sp. CPCC 200497, a quinomycins producer. Methods Silica gel TLC, medium-low-pressure preparative chromatography and reversed phase HPLC were used to analyze the Et OAc extract of agar culture of Streptomyces sp. CPCC 200497 or purify compounds of interests. LC-MS and NMR were employed to identify the target compounds. Results Bohemamine and bohemamine B, two pyrrolizidine alkaloids were found in ethyl acetate extracts of fermentation from Streptomyces sp. CPCC 200497 and characterized for their structures. Conclusion Bohemamines are identified for the first time as secondary metabolites from Streptomyces sp. CPCC 200497,a quinomycins producing strain. Their structures are established on the basis of extensive spectroscopic data. This is the first report of bohemamines in China.

关 键 词：吡咯里西啶类生物碱 波西米胺 醌霉素 

分 类 号：R915[医药卫生—微生物与生化药学]