益生菌促进阿卡波糖降低糖尿病小鼠餐后血糖的研究  

Study on the reduction of postprandial blood glucose with acarbose promoted by four-combination probiotics in diabetic mice

作　　者：钟丹[1] 殷瑜[1,2] 戈梅[2] 钱秀萍[1] ZHONG Dan YIN Yu GE Mei QIAN Xiu-ping(School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China Shanghai Laiyi Center for Biopharmaceutical R＆D, Shanghai 201203, China)

机构地区：[1]上海交通大学药学院,200240 [2]上海来益生物药物研究开发中心有限责任公司,201203

出　　处：《中国医药生物技术》2016年第5期441-445,共5页Chinese Medicinal Biotechnology

摘　　要：目的研究四联益生菌对阿卡波糖降低糖尿病小鼠餐后血糖作用的影响。方法高脂饲料喂养4周的小鼠腹腔注射链脲佐菌素（STZ）造模,随机血糖大于16.7 mmol/L即视为糖尿病造模成功。造模成功的小鼠灌胃四联益生菌（嗜酸乳杆菌、鼠李糖乳杆菌、长双歧杆菌及地衣芽孢杆菌）各109 cfu/d,3周后,考察益生菌是否具有促进阿卡波糖降低糖尿病小鼠餐后血糖的作用。并通过检测小鼠血清胆固醇、甘油三酯、胰岛素及胰高血糖素样肽-1（GLP-1）水平,研究益生菌对小鼠糖尿病的改善作用。结果单独给以低剂量阿卡波糖30 mg/kg或四联益生菌均无明显降低餐后血糖的作用,但两者联合给药组小鼠餐后1~2 h血糖值明显低于的糖尿病组及单独给以阿卡波糖或益生菌组。四联益生菌组小鼠血清TG水平明显低于糖尿病组,而GLP-1水平则明显高于糖尿病组,两组间血清胆固醇及胰岛素无显著性差异。结论四联益生菌具有促进阿卡波糖降低糖尿病小鼠餐后血糖的作用,这种促进作用可能跟益生菌改善甘油三酯及GLP-1的水平有关。Objective To evaluate the effect of four-combination probiotics on reducing postprandial blood glucose of acarbose in diabetic mice. Methods The mice were fed with diet enriched with high glucose and high fat, and after 4 weeks streptozotocin（STZ） was injected intraperitoneally. The criteria for the successful modelling was random blood sugar（RBS） aboved the cutoff value（16.7 mmol/L）. The probiotics group mice were given orally four-combination probiotics（Lactobacillus acidophilus, L. rhamnosus, Bifidobacterium longum, Bacillus icheniformis）, after 3 weeks their promotion on hypoglycemic activities to acarbose in diabetic mice were assayed. At the end of the experimental period, triglyceride, total cholesterol, insulin and GLP-1 in the plasma were determined.Results Neither four-combination probiotics nor 30 mg/kg acarbose alone showed obvious hypoglycemic activity in mice. But when 30 mg/kg acarbose combined with probiotics was administrated, postprandial blood glucose of probiotics group mice was reduced more than that of the control group with no drug. Plasma triglyceride was significantly lower in probiotics group than that in diabetic group, while the GLP-1 levels of probiotic group was significantly higher than that of diabetic group. The levels of cholesterol and insulin were not significantly different between the two groups. Conclusion Hypoglycemic function of acarbose is significantly promoted by probiotics, probably because of the improvement of plasma GLP-1 and triglyceride levels.

关 键 词：糖尿病 阿卡波糖 小鼠 肥胖 益生菌 胰高血糖素样肽1 

分 类 号：R587.1[医药卫生—内分泌]