大鼠上橄榄复合体中枢听觉信息处理相关蛋白的研究  

A study on the proteins of superior olivary complex related to central auditory processing in SD rats

作　　者：徐莹 李宛桐 李晓璐 

机构地区：[1]南京医科大学第一附属医院耳鼻咽喉科,WHO预防聋和听力减退合作中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2016年第9期1068-1071,1078,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省“科教兴卫工程”医学重点人才项目(RC2011073);江苏省科技厅国际合作项目(BZ2011043);江苏省人力资源和社会保障厅“六大人才高峰”项目(2009075)

摘　　要：目的 :同位素相对标记和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术分析大鼠上橄榄复合体(superior olivary complex,SOC)胞膜差异蛋白。方法:从P60健康的雄性Sprague Dawley(SD)大鼠的脑组织中,分离出耳蜗核(cochlear nuclear complex,CN)、SOC和下丘(inferior colliculus,IC),用iTRAQ技术检测SOC区域胞膜蛋白并分析其差异表达的蛋白质。以剩余脑组织(Rest)作为对照。结果:质谱分析共鉴定到1 937种膜蛋白质,核团间两两比较,确定SOC有14种区域特异蛋白。Gene ontology(GO)功能分析提示丙氨酸-t RNA连接酶(alanyl-t RNA synthetase,Aars)、双官能谷氨酰-脯氨酸-t RNA合成酶(glutamyl-prolyl-t RNA synthetase,Eprs)、α-内连蛋白(α-internexin,Ina)和溶质载体44A1(solute carrier 44A1,Slc44A1)等蛋白主要参与神经系统发育、神经传递和突触可塑性,其余蛋白与能量代谢有关。结论:iTRAQ技术分析SOC胞膜差异蛋白,鉴定到Aars、Eprs、Ina、Slc44A1等蛋白参与中枢听觉信息处理,为进一步研究中枢听觉处理障碍提供了理论依据。Objective： The proteomic analysis of isobaric tags for relative and absolute quantitation（iTRAQ） technique was performed to analyze the differential plasma membrane proteins of superior olivary complex（SOC） of SD rats. Methods： The cochlear nuclear complex（CN）,SOC and inferior colliculus（IC） were isolated from P60 healthy male Sprague Dawley（SD） rats,the rest of the brain was used as control. Then the iTRAQ technique was used to detected and identified the differential plasma membrane protein expression in these regions. Results： A total of 1 937 plasma membrane proteins showed significantly different expression,between two regions. We found 14 region-typical proteins in SOC. Gene ontology（GO） functional analysis showed that alanyl-t RNA synthetase（Aars）,glutamyl-prolyl-t RNA synthetase（Eprs）,α-internexin（Ina） and solute carrier 44A1（Slc44A1） mignt be involved in neural development,neurotransmission,synaptic remolding, and affect the neurotransmission of auditory neurons. Conclusion： The quantitative comparison of protein screened reveals that several interesting candidate proteins, including Aars,Eprs,Ina,Slc44A1, are related to central auditory processing, which provides a theoretical basis for further study of auditory processing disorder.

关 键 词：中枢听觉信息处理 ITRAQ 蛋白质组学 

分 类 号：R393[医药卫生—基础医学]