丁苯酞胶囊对脑缺血再灌注损伤大鼠脑组织Bim蛋白表达的影响  

Effects of dl-3-n-butylphthalide capsule on the expression of Bim protein of rats with focal cerebral ischemia-reperfusion

作　　者：杨小霞[1] 李国前[1] 王杰华[1] 洪诸权[1] 潘莹[1] 

机构地区：[1]福建医科大学附属泉州第一医院神经内科,福建泉州362000

出　　处：《中国临床药理学杂志》2016年第20期1878-1880,共3页The Chinese Journal of Clinical Pharmacology

基　　金：泉州市卫生局科研基金资助项目;泉州市科技计划基金资助项目(2013Z62)

摘　　要：目的观察丁苯酞(NBP)对大鼠脑缺血再灌注损伤后脑组织Bim蛋白表达的影响。方法将24只雄性SD大鼠随机分为假手术组、模型组和实验组,每组8只。模型组和实验组用线栓法制作大鼠大脑中动脉脑缺血再灌注模型,假手术组接受相似手术处理,但是不插入线栓。实验组于大脑中动脉栓塞后15 min内灌胃予以80 mg·kg^(-1)丁苯酞,给药一次;模型组和假手术组予以相同体积的食用麻油,给药一次。用TUNEL染色法原位检测各组大鼠缺血侧皮质区神经元细胞凋亡情况,用免疫组织化学法和Western blot法检测各组大鼠缺血侧皮质区Bim蛋白表达情况。结果与假手术组(16.36±3.29)相比,模型组和实验组缺血侧皮质区神经元细胞凋亡程度分别为(89.42±12.68)和(56.38±8.71),差异均有统计学意义(P<0.05)。与假手术组(0.13±0.03)相比,模型组和实验组缺血侧皮质区Bim蛋白表达水平分别为(0.82±0.14)和(0.64±0.08),差异均有统计学意义(P<0.05)。实验组和模型组的缺血侧皮质区神经元细胞凋亡程度、Bim蛋白表达水平比较差异均有统计学意义(P<0.05)。结论丁苯酞能抑制大鼠脑缺血再灌注损伤后的神经细胞凋亡,其机制可能与抑制Bim蛋白的表达有关。Objective To observe the influence of dl-3-n-butylphthalide on the expression of Bim protein in rats with focal cerebral ischemia-reperfusion.Methods Twenty-four male SD rats were randomly assigned into sham operation group（sham group）,model group and test group.The middle cerebral artery occlusion reperfusion models were made by the suture method for the rats in model sham groups and test group（ischemia for 2 h,and reperfusion for 24 h）.The rats in sham group were taken the similar operation without suture.The rats in test group were lavaged with NBP 80 mg·kg^（-1）once after making a model,and the rats in model and sham groups were lavaged with the same volume of edible oil.TUNEL staining was used to observe neuron apoptosis in the ischemic region of the brain tissue.The level of Bim protein expression was measured by immunohistochemistry and Western blot.Results Compared with sham group（16.36 ± 3.29）,TUNEL positive cells were（89.42 ± 12.68） and（56.38 ± 8.71） in model group and test group,with significant difference（P 0.05）.Compared with sham group（0.13 ± 0.03）,Bim protein were（0.82 ± 0.14） and（0.64 ±0.08） in model group and test group,with significant difference（P 0.05）.There were significant difference with TUNEL positive cells and Bim protein in model and test group（P 0.05）.Conclusion dl-3-n-butylphthalide has the protective effects on cerebral ischemia-reperfusion injury in rats,partly through decreasing Bim protein synthesis in brain.

关 键 词：丁苯酞 脑缺血再灌注 凋亡 BIM蛋白 

分 类 号：R972[医药卫生—药品]