普瑞巴林胶囊联合吴茱萸碱注射剂对神经病理性疼痛大鼠疼痛行为学和炎性细胞因子的影响  

Effect of pregabalin capsule combined with evodiamine injection on inflammatory cytokines and pain behavior in rats with neuropathic pain

作　　者：章玲宾[1] 潘晨[2] 许晓雅[1] 王昌雄[1] 张宁[1] 游敏吉[1] 陈琴[1] 陈佳琪[3] 

机构地区：[1]丽水市人民医院麻醉科,浙江丽水323000 [2]丽水市人民医院药学部,浙江丽水323000 [3]丽水市人民医院检验科,浙江丽水323000

出　　处：《中国临床药理学杂志》2016年第20期1890-1893,共4页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省中西医结合学会临床药学科研基金资助项目(2014LYZD005));浙江省医学会临床科研基金资助项目(2010ZYC-A48);丽水市高层次人才基金资助项目(2014RC35)

摘　　要：目的研究普瑞巴林联合吴茱萸碱对神经病理性疼痛大鼠疼痛行为学和炎性细胞因子的影响。方法通过暴露大鼠左侧L5脊神经并结扎,建立脊神经结扎(SNL)神经病理痛模型模型。按随机数字表法随机分为5组:假手术组,模型组,西药-实验组,中药-实验组和联合实验组。建立SNL模型7 d后,西药-实验组腹腔注射普瑞巴林5 mg·kg^(-1),中药-实验组腹腔注射吴茱萸碱注射液5 mg·kg^(-1),联合实验组腹腔注射普瑞巴林与吴茱萸碱的复合物5 mg·kg^(-1),假手术组和模型组腹腔注射生理盐水2 m L·kg^(-1),连续给药7 d。用手控电子记录仪以单盲法记录大鼠神经损伤侧于5 min内的抬足次数并以此作为持续性疼痛的检测指标。经主动脉取血3 m L,用酶联免疫吸附法检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的水平。结果于术后14 d的持续性疼痛(CIPP),西药-实验组为(9.46±1.50)次、中药-实验组为(9.31±1.62)次和联合实验组(4.32±1.64)次,较模型组的(23.31±1.66)次,差异有统计学意义(P<0.05)。其中,联合实验组较西药-实验组和中药-实验组差异也有统计学意义(P<0.05)。给药14 d后TNF-α水平,西药-实验组为(84.67±10.11)ng·L^(-1),中药-实验组为(82.47±9.54)ng·L^(-1)和联合实验组为(68.54±7.65)ng·L^(-1),较模型组的(101.75±15.46)ng·L^(-1)差异有统计学意义(P<0.01或<0.05);IL-6水平:西药-实验组为(25.55±4.63)ng·L^(-1),中药-实验组为(23.87±3.67)ng·L^(-1)和联合实验组为(20.21±4.23)ng·L^(-1),较模型组的(32.98±6.64)ng·L^(-1)差异有统计学意义(P<0.01或P<0.05)。结论普瑞巴林、吴茱萸碱均对神经病理性疼痛有镇痛作用,且两者联合使用镇痛效果更好。Objective To observe the effect of pregabalin and evodiamine on pain behaviors of neuropathic pain and inflammatory cytokines in rats.Methods Rats were exposed to the left L5 spinal nerve and ligation to establish the spinal nerve ligation（SNL） model.According to the random number table method were randomly assigned for 5 groups：sham operation group,model group,medicine-test group,herb-test group and united-test group.Established in SNL 7 days after the start of medicine-test group were treated with intraperitoneal injection of pregabalin 5 mg·kg^（-1）,herb-test group were intraperitoneally injected with evodiamine 5 mg·kg^（-1）,united-test group received intraperitoneal injection of pregabalin-evodiamine complexes 5 mg · kg^（-1）,shamoperation group and model group by intraperitoneal injection of physiological saline 2 m L·kg^（-1）,continuous administration for 7 days.From the beginning of the experiment until 7 days after the administration,the foot lift as a factor of cold induced sustained pain（CIPP） was observed after the mechanical constriction of the injured side of 5 minutes.After 7 days administration,the levels of interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α） were detected by ELISA.Results After 14 days treatment,Compared to model group on the CIPP with（23.31 ± 1.66） times,the CIPP in medicine-test group with（9.46 ± 1.5） times,herb-test group with（9.31 ± 1.62） times and united-test group with（4.32 ± 1.64） times were significant reduced（P 0.05）,but were united-test group was significant lower than the medicine-test group and herb-test group（P 0.05）.After 14 days treatment,Compared to model group on the TNF-α with（101.75 ± 15.46） ng · L-1,the TNF-α in medicine-test group with（84.67 ± 10.11） ng·L^（-1）,herb-test group with（82.47 ± 9.54） ng·L^（-1）and united-test group with（68.54 ± 7.65） ng·L^（-1）were significant reduced（P 0.01 or P 0.05）.After 14 days treatment,compare to model group on the IL-6

关 键 词：神经病理性疼痛 普瑞巴林 吴茱萸碱 疼痛行为学 炎性细胞因子 

分 类 号：R971.1[医药卫生—药品]