GPER激动剂对发情周期小鼠卵巢EGFR表达的影响  

Effect of G-protein-coupled estrogen receptor(GPER)agonist on expression of EGFR in mice ovary during estrous cycle

作　　者：王璐[1] 刘红改[1] 于得水[1] 李晶[1] 刘慧[1] 赵慧英[1] WANG Lu LIU Honggai YU Deshui LI Jing LIU Hui ZHAO Huiying(College of Veterina ry Medicine, North west A ＆ F University, Yangling, Shaa nxi 712100, China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2016年第9期22-26,34,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：抗病转基因牛新品种培育重点专项基金项目(2009ZX08007)

摘　　要：【目的】探讨G蛋白耦联雌激素受体(G-protein-coupled estrogen receptor,GPER)激动剂对卵巢表皮生长因子受体(Epidermal growth factor receptor,EGFR)表达的影响,为阐明GPER和EGFR在卵巢功能维持中的作用奠定基础。【方法】取间情期小鼠,将其分为40,200,1 000ng/只G-1腹腔注射组和对照组(腹腔注射等体积生理盐水),每组20只,各组小鼠进行相应的处理。注射G-1后,依次取发情前期、发情期、发情后期和间情期小鼠各5只,脱颈处死,迅速取出卵巢,采用免疫组织化学SP法和实时定量RT-PCR法,研究不同剂量GPER激动剂G-1对发情周期小鼠卵巢EGFR分布及其mRNA表达的影响。【结果】EGFR在小鼠卵巢的各级卵泡、黄体和间质中均有表达,且在卵泡颗粒细胞和卵母细胞中表达最明显,在黄体和间质中的表达较微弱。同一发情时期卵巢中,EGFR的相对表达量随注射G-1剂量的增加而升高,除发情后期外,其他3个时期以1 000ng/只G-1注射组均极显著高于对照组(P<0.01);间情期和发情前期则以200ng/只G-1注射组显著高于对照组(P<0.05)。各组发情周期小鼠卵巢中EGFR mRNA表达量的变化规律与EGFR相对表达量的表现基本一致。【结论】G-1对发情周期卵巢中EGFR的表达有一定的上调作用,提示GPER可介导雌激素经EGFR信号级联参与卵巢周期性生殖活动和卵泡生长发育的调节。【Objective】This study explored the effect of G-protein-coupled estrogen receptor（GPER）agonist on the expression of epidermal growth factor receptor（EGFR）in ovary to lay foundation for clarifying the functional relationship between GPER and EGFR.【Method】The diestrus mice were divided into40,200 and 1 000 ng dose of G-1intraperitoneal injection groups and the control group（intraperitoneal injection of normal saline）.Each group included twenty mice.Five mice were selected in proestrous,estrous,metestrous and diestrous and sacrifying periods after injection of G-1and their ovaries were removed quickly.Immunohistochemical SP method and real-time quantitative RT-PCR were used to study the distribution of EGFR and expression of EGFR mRNA in mice ovary during the estrus cycle.【Result】EGFR distributed in mice ovarian follicles,the corpus luteum and stroma with the largest expression in granulosa cells and oocytes and weak expression in corpus luteum and stroma.The expression of EGFR increased with the in-creasing dose of G-1during the same period.Except for metestrous,the EGFR expression in ovary of 1 000 ng G-1group was significantly higher than the control in other three periods（P〈0.01）.The expression in ovary of 200 ng G-1group in diestrus and proestrus periods was significantly higher than the control（P〈0.05）.The expression of EGFR mRNA was consistent with the relative expression of EGFR in each group.【Conclusion】G-1upregulated the expression of EGFR in ovary during estrous cycle.Suggesting GPER can mediate estrogen involved in regulating cyclical ovarian reproductive activity and follicular development by EGFR signaling cascade.

关 键 词：G蛋白耦联雌激素受体 表皮生长因子受体 发情周期卵巢 

分 类 号：Q492.5[生物学—生理学]