CD137表达与慢性乙型肝炎患者肝脏免疫病理损伤程度的相关性研究  

Relationship between the CD137 expression and immunopathological injury degree of liver in patients with chronic hepatitis B

作　　者：王博[1] 吴俊英[1,2] 徐超[3] WANG Bo WU Jun-ying XU Chao(Lab of Clinical Laboratory Diagnostics,Bengbu Medical College,Bengbu Anhui 233030 Department of Laboratory Medicine, Bengbu Medical College, Bengbu Anhui 233030 Department of Infectious Diseases, The 123rd Hospital of PLA ,Bengbu Anhui 233000, China)

机构地区：[1]蚌埠医学院临床检验诊断学实验中心,安徽蚌埠233030 [2]蚌埠医学院医学检验系,安徽蚌埠233030 [3]中国人民解放军第123医院传染科,安徽蚌埠233000

出　　处：《蚌埠医学院学报》2016年第9期1125-1128,共4页Journal of Bengbu Medical College

基　　金：安徽省高校省级自然科学研究重点项目(KJ2012A195);蚌埠医学院研究生科研创新计划(byycx1408)

摘　　要：目的:探讨CD137表达与慢性乙型肝炎(chronic hepatitis B,CHB)患者肝脏免疫病理损伤程度的相关性。方法:采用ELISA法检测受试者外周血中CD137L的水平;免疫荧光法检测肝组织中CD137的表达;Western blot法检测肝组织中CD137和CD28蛋白水平表达;荧光定量PCR检测肝组织中CD137和CD28 mRNA的表达。结果:重度CHB组患者血清CD137L的表达水平均明显高于健康对照组、轻度和中度CHB组患者(P<0.01)。肝组织中CD28 mRNA在中度CHB组和重度CHB组患者的表达水平均较健康对照组及轻度CHB组患者明显升高(P<0.01)。重度CHB组患者CD137 mRNA表达水平均较健康对照组、轻度CHB组和中度CHB组患者显著升高(P<0.01)。肝组织中CD28蛋白在中度CHB组和重度CHB组患者的表达水平均较健康对照组及轻度CHB组患者明显升高(P<0.01)。重度CHB组患者CD137蛋白表达水平均较健康对照组、轻度CHB组和中度CHB组患者中CD137蛋白表达水平显著升高(P<0.01)。结论:CD137的活化参与CHB患者肝组织免疫病理损伤过程,与其损伤程度有相关性。Objective：To investigate the correlation of the expression of CD137 and immunopathological injury degree of liver in patients with chronic hepatitis B （CHB）. Methods：The levels of CD137 in peripheral blood and liver tissue were measured by ELISA and immunofluorescent assay,respectively. The levels of the protein and mRNA expressions of CD137 and CD28 in liver tissue were detected using Western blot and qPCR,respectively. Results：The level of serum CD137 in severe CHB group was significantly higher than that in healthy control group,mild and moderate CHB groups（P 〈0.01 ）. The expression levels of CD28 mRNA in liver tissue in moderate and severe CHB groups were higher than that in healthy control group and mild CHB group（P 〈 0.01 ）. The expression level of CD137 mRNA in liver tissue in severe CHB group was significantly higher than that in healthy control group,mild and moderate CHB groups（P 〈0.01 ）. The expression levels of CD28 protein in liver tissue in moderate and severe CHB groups were higher than that in healthy control group and mild CHB group （P 〈 0.01 ）. The expression level of CD137 protein in liver tissue in severe CHB group was higher than that in healthy control group, mild and moderate CHB groups（P 〈 0.01 ）. Conclusions：The activation of CD137 involves in the immunopathological injury process of liver in CHB patients, and is related to the degree of liver.

关 键 词：慢性乙型肝炎 CD137 免疫病理损伤 肝损伤 

分 类 号：R392.32[医药卫生—免疫学]