正交设计在观赏石斛种胚离体培养中的应用  

Application of Orthogonal Design in Embryo Culture of Ornamental Dendrobium

作　　者：龚建英[1] 王华新[1] 陈宝玲[1] 陈尔[1] 杨舒婷[1] 苏莉花[1] GONG Jian-ying WANG Hua-xin CHEN Bao-lin CHEN Er YANG Shu-ting SU Li-hua(Guangxi Forestry Research Institute, Nanning 530002, Chin)

机构地区：[1]广西壮族自治区林业科学研究院,南宁530002

出　　处：《广西林业科学》2016年第3期306-310,共5页Guangxi Forestry Science

基　　金：广西林业科技项目(桂林科字[2013]第5号);广西林业科技项目(桂林科字[2010]第1号);广西林科院基本科研业务费专项(林科201425号)

摘　　要：利用正交设计方法 L9（34）,研究石斛种类、MS用量、有机添加物种类以及6-BA浓度等4个因子对观赏石斛种胚离体培养的影响,结果表明：6-BA浓度是影响观赏石斛种胚萌动时间的主要因子,其次是石斛种类,最后是有机添加物种类和MS用量。观赏石斛种胚萌动最优的水平组合为金钗石斛＋MS＋香蕉＋6-BA0.2 mg/L,种胚最快萌动时间为9.6 d。MS用量和6-BA浓度是影响观赏石斛种胚萌发率的主要因子,其次是有机添加物种类,最后是石斛种类。观赏石斛种胚萌发率最优的水平组合为重唇石斛＋MS＋土豆＋6-BA 0.2mg/L,种胚萌发率达100%。The impacts of such four factors as Dendrobium species, MS content, organic additives types and 6 - BA concentration on embryo culture of ornamental Dendrobium were studied by the method of L9 （ 34 ） orthogonal design. The results showed that 6 - BA was the main factor influencing embryo germina- tion time of ornamental Dendrobium, followed by Dendrobium species and organic additives, with MS as the last impact factors. The best combination for embryo germination time was D. nobile ＋ MS ＋ banana ＋ 6 - BA0. 2 mg/L, with the fastest embryo germination time of 9.6 days. MS and 6 - BA were the main factors influencing embryo germination rate, followed by organic additives, and Dendrobium species as the last impact factor. The best combination for embryo germination rate was D. hecoglossum ＋ MS ＋ potato ＋ 6 - BA0. 2 mg/L with 100% embryo germination rate.

关 键 词：观赏石斛 种胚培养 正交设计 

分 类 号：S723.1[农业科学—林木遗传育种] S628.31[农业科学—林学]