NOD鼠体内CD_4^+NKG2D^+T细胞与CD_8^+T细胞间关系  

Relationship between CD_4^+NKG2D^+T and CD_8^+T cells in NOD rats

作　　者：王宇航[1] 马旻轩 徐娅雯[1] 李凯[1] 潘兴元[1] 钱莉[1] 龚卫娟[1] 季明春[1] 

机构地区：[1]扬州大学医学院病原生物学和免疫学教研室,江苏扬州225001

出　　处：《实用临床医药杂志》2016年第19期5-8,共4页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金(30771955)

摘　　要：目的探讨NOD鼠体内CD_4^+NKG2D^+T细胞与CD_8^+T细胞间关系。方法动态监测NOD鼠外周血中CD_4^+NKG2D^+T及CD8+NKG2D^+T细胞在不同周龄频率。利用IGRP206-214特异性CTL清除的NOD鼠,对其外周血中CD_4^+NKG2D+/CD_4^+T及CD8+NKG2D+/CD_8^+T细胞频率进行分析。将磁珠分选所得CD_4^+NKG2D^+T细胞分别与经CFSE标记的纯CD_4^+NKG2D-T细胞、CD_8^+T细胞共培养4 d,检测体系中CD_4^+NKG2D-T细胞、CD_8^+T细胞CFSE的荧光强度变化。结果 NOD鼠外周血CD_4^+NKG2D+/CD_4^+T及CD8+NKG2D+/CD_8^+T细胞频率均随其1型糖尿病疾病进程发展逐渐升高,且二者之间呈正相关。IGRP206-214特异性CTL清除的NOD鼠,外周血中CD_4^+NKG2D+/CD_4^+T细胞频率随CD8+NKG2D+/CD_8^+T细胞频率降低显著下降。与单独培养的CD_4^+NKG2D-T细胞相比,加入CD_4^+NKG2D^+T细胞的培养体系中,CD_4^+NKG2D-T细胞增殖加快。但CD_4^+NKG2D^+T细胞对CD_8^+T增殖作用不明显。结论 NOD鼠体内存在着一群与1型糖尿病疾病进程正相关的CD_4^+NKG2D^+T细胞,且该群细胞极有可能是通过直接作用于CD_4^+NKG2D-T细胞而间接对CD_8^+T细胞产生作用。Objective To explore the relationship between CD4^＋NKG2D^＋T and CD8^＋T cells in NOD rats. Methods The frequency of CD4^＋NKG2D^＋T and CD8^＋NKG2D^＋T cells in peripheral blood of NOD rats in different weeks were dynamically monitored. By using IGRP206- 214- SAP NOD rats,the frequencies of CD4^＋NKG2D^＋/ CD4^＋T and CD8^＋NKG2D^＋/ CD8^＋T cells in peripheral blood were analyzed. CD4^＋NKG2D^＋T cells was purified and CFSE CD4^＋NKG2D^-T cells or CD8^＋T were co-cultured 4 days were marked. The CFSE fluorescence intensity change of CD4^＋NKG2D^-T cells or CD8^＋T cells were detected. Results The frequency of CD4^＋NKG2D^＋/ CD4^＋T and CD8^＋NKG2D^＋/ CD8^＋T cells in peripheral blood of female NOD rats during the development of type 1 diabetes disease process gradually increased,and they were positively correlated to the linear.In the NOD rats with IGRP206- 214-SAP,the frequency of CD4^＋NKG2D^＋/ CD4^＋T cells in peripheral blood was reduced by CD8^＋NKG2D^＋/ CD8^＋T cells reduction. Compared with alone CD4^＋NKG2DT cells,the proliferation of CD4^＋NKG2D^-T cells was speeded up in cell culture system incubated with CD4^＋NKG2D^＋T. CD4^＋NKG2D^＋T cells is no direct effect on the CD8^＋T cell. Conclusion An increased CD4^＋NKG2D^＋T cells subsets in NOD mice might have an effect on CD8^＋T cells through activation of CD4^＋NKG2D^-T cells.

关 键 词：CD4^+NKG2D^+T细胞 CD8^+T细胞 1型糖尿病 NOD鼠 

分 类 号：R392.2[医药卫生—免疫学]