马氏珠母贝贝壳珍珠质层厚度性状与微卫星标记的相关性分析  

Analysis of Correlationship Between the Thickness of Shell Inner Nacreous Layer and Microsatellite Markers in Pinctada fucata martensii

作　　者：战欣[1] 陈琼[1] 顾志峰[1] 石耀华[1] 王爱民[1] ZHAN Xin CHEN Qiong GU Zhifeng SHI Yaohua WANG Aimin(Ministry of Education Key Laboratory of Tropic Biological Resources/ Hainan Key Laboratory of Tropical Hydrobiological Technology, College of Marine Science, Hainan University, Haikou 570228, Chin)

机构地区：[1]海南大学海洋学院/热带生物资源教育部重点实验室/海南省热带水生生物技术重点实验室,海口570228

出　　处：《热带生物学报》2016年第3期290-295,共6页Journal of Tropical Biology

基　　金：国家重点基础研究发展计划(2010CBl26405);国家高技术研究发展计划(2012AA10A414);海南省青年基金项目(313033);海南大学科研启动基金资助项目(kyqdl216);海南大学青年基金资助项目(qnjjl227)

摘　　要：运用科研型光学相干断层扫描仪测量了341个马氏珠母贝贝壳内表面珍珠质层的厚度,同时分析了这341个贝壳内表面珍珠质层厚度性状与5个微卫星标记的相关性。结果表明:其中4个微卫星位点(2PM206,4PM34,4PM50和PM6)与检测的珠层厚度性状的相关性达到显著水平(P<0.05);2PM206标记173/173基因型个体的贝壳珍珠质层厚度的平均值、4PM34标记189/189基因型个体对应的性状平均值、4PM50标记201/205基因型个体的珍珠质层厚度值和PM6标记260/266基因型个体对应的珍珠层厚度平均值较同一标记的其他基因型均为最大值;4PM50标记中含有209 bp等位基因个体的珍珠层厚度平均值均低于其他基因型,209 bp等位基因可能与贝壳珍珠质层厚度性状之间存在负相关关系。Thickness of shell inner nacreous layer was measured by using optical coherence tomography in 341 pearl oysters. Correlativity between five microsatellite markers and thickness of shell inner nacreous layer was calculated by one-way analysis of variance. Four out of the five markers（ 2PM206,4PM34,4PM50 and PM6）were correlated with the test trait（ P 0. 05）. It is shown that oysters with 173 /173 genotype in locus 2PM206,with 189 /189 genotype in locus 4PM34,with genotype 201 /205 in locus 4PM50 and 260 /266 genotype in locus PM6 had significantly higher thickness of shell nacreous layer than other genotype individuals（ P 0. 05）. Individuals with allele 209 had lower values of the tested traits than all the other individuals with alleles in 4PM50 marker,and hence allele 209 may have negative correlation with the thickness of shell nacreous layer（ P 0. 05）.

关 键 词：马氏珠母贝 生物矿化 珍珠质层 相关性 微卫星 

分 类 号：S968.316.1[农业科学—水产养殖]