早期输注20%脂肪乳剂对大鼠急性胰腺炎的影响  

Influence of intravenous injection of 20% fat emulsion at early stage on acute pancreatitis of rat model

作　　者：赵艳梅[1] 菅志远[1] 

机构地区：[1]湖北省十堰市太和医院(湖北医药学院附属医院)肝胆胰外科诊疗中心,湖北十堰442000

出　　处：《中国现代医学杂志》2016年第19期23-27,共5页China Journal of Modern Medicine

基　　金：湖北省十堰市科技项目(No:ZD2011008)

摘　　要：目的探讨早期经外周静脉注射20%脂肪乳剂对大鼠急性胰腺炎(AP)严重程度的影响及其作用机制。方法以SD大鼠为研究对象,将其随机分为A组(模型对照组)、B组(脂肪乳剂干预组)和C组(AP生理盐水组),每组15只,建立大鼠AP模型。B组在建模手术后1、3和9 h经外周静脉输注20%脂肪乳剂,C组在建模手术后1、3和9 h经外周静脉注射生理盐水。检测大鼠6、12和24 h血清三酰甘油、胆固醇及微循环相关因子的变化,并观察大鼠AP严重程度的病理变化。结果成功建立大鼠AP模型。B、C组6 h的病理学评分比较,差异无统计学意义;12和24 h的病理学评分比较,差异有统计学意义。B组6 h的血清三酰甘油、胆固醇、C-反应蛋白(CRP)、血管紧张素-Ⅱ(AngⅡ)及血栓素B2(TXB2)水平高于C组。B组12 h的血清三酰甘油、胆固醇、CRP及TXB2水平高于C组。B组24 h的血清三酰甘油、胆固醇、CRP、AngⅡ及TXB2水平高于C组。结论大鼠AP早期经外周静脉注射脂肪乳剂可使大鼠的血脂升高,其可能通过影响微循环使大鼠AP程度加重。Objective To observe the effect of intravenous injection of 20% fat emulsion at early stage on the severity of the rat acute pancreatitis（AP）. Methods Experimental pancreatitis model of SD rats were induced by an retro-pancreatic duct injection of Sodium Taurocholate, and the experimental rats were divided into model group（group A）, study group（group B） and control group（group C） with 15 rats in each group.At the 1st, 3rd and 9th hour after the modeling surgery of acute pancreatitis, the rats in the group B were injected with the 20% fat emulsion through the caudal vein, while those in the group C were injected with normal saline. At the 6th, 12 th and 24 th hour after the modeling operation, serum triglyceride, cholesterol and the factors related to the microcirculation were examined by ELISA, and the score of the pathological grade of the AP severity was calculated. Results The rat AP model was successfully induced. There were significant differences in the pathological grade score between the group B and the group C at the 12 th and 24 th hour but not at the 6th hour after the modeling operation. At the 6th hour after the modeling operation, the serum levels of triglyceride, cholesterol, C-reaction protein（CPR）, angiotensin Ⅱ（Ang Ⅱ） and thromboxane B2（TXB2） in the group B were significantly higher than those in the group C. At the 12 th hour, the serum levels of triglyceride, cholesterol, CPR and TXB2 in the group B were obviously higher than those in the group C. And at the 24 th hour after the operation, the serum levels of triglyceride, cholesterol, CPR, Ang Ⅱand TXB2 in the group B were significantly higher than those of the group C. Conclusions Peripheral intravenous injection of 20% fat emulsion at early stage could induce rat hyperlipidemia, which could increase the severity of rat acute pancreatitis possibly by affecting pancreatic microcirculation.

关 键 词：急性胰腺炎 血脂 微循环 

分 类 号：R657.51[医药卫生—外科学]