固相萃取-表面增强拉曼光谱法测定奶粉中三聚氰胺  

Determination of Melamine in Milk Powder by Solid-phase Extraction Coupled with Surface-enhanced Raman Spectroscopy

作　　者：陈小曼[1] 雷皓宇 胡玉玲[1] 李攻科[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275

出　　处：《分析测试学报》2016年第10期1343-1346,共4页Journal of Instrumental Analysis

基　　金：国家重大科学仪器设备开发专项(2011YQ03012409);国家自然科学基金(21575168;21277176;21475153);广东省自然科学基金(015A030311020);广东省公益研究与能力建设专项(2015A030401036);广州市科技计划项目(201604020165)

摘　　要：建立了固相萃取-表面增强拉曼光谱法（SPE-SERS）测定奶粉中三聚氰胺的分析方法。待测物经乙腈提取、活性碳吸附及氢氧化钠溶液洗脱后进行SERS检测。该方法的线性范围为0.005 0~1.6 mg/L,检出限为0.100 mg/kg,回收率为75.3%~125%,相对标准偏差（RSD,n=5）不大于9.3%。该法灵敏度高、杂质干扰小、结果准确可靠,能满足奶粉中三聚氰胺的快速检测,在奶粉质量监控方面具有良好的应用潜力。A new analytical method was developed for the determination of melamine in milk powder by solid-phase extraction coupled with surface-enhanced Raman spectroscopy. The melamine in sam- ple was extracted with acetonitrile, then adsorbed with activated carbon and eluted with sodium hy- droxide solution. The results showed that the linear range for melamine was in the range of 0. 005 0 - 1.6 rag/L, with a limit of detection of 0. 100 mg/kg. While the method was applied in the analysis of melamine in milk powder, the recoveries for melamine were in the range of 75.3% - 125% , and the relative standard deviations （n = 5 ） were not more than 9.3% . With a remarkable effect in remo- ving impurities, this method is accurate, reliable and sensitive in the rapid determination of mela- mine in milk powder, showing a great potential in monitoring the quality of milk powder.

关 键 词：表面增强拉曼光谱法 固相萃取 奶粉 三聚氰胺 

分 类 号：O657.3[理学—分析化学] TS252.51[理学—化学]