HPLC法测定当归芍药散中芍药苷的含量  

Content Determination of Peoniflorin in Danggui Shaoyao Powder by HPLC

作　　者：周珺[1] 王登[2,3] 张黎[1] 张锦[1,2] 李茂星[1] 张汝学[1,2] 贾正平[1] 

机构地区：[1]兰州军区兰州总医院全军高原环境损伤防治重点实验室/国家中医药管理局临床中药学重点学科,兰州730050 [2]甘肃中医学院药学院,兰州730000 [3]西安大兴医院药剂科,西安710016

出　　处：《中国药房》2016年第30期4255-4257,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81173620)

摘　　要：目的：建立测定当归芍药散中芍药苷含量的方法,以期为控制该制剂的质量提供参考。方法：采用高效液相色谱法。色谱柱为Symmetry C_（18）,流动相为乙腈-水（含0.1%磷酸）（14∶86,V/V）,流速为1.0 ml/min,检测波长为230 nm,柱温为20℃,进样量为20μl。结果：芍药苷检测质量浓度线性范围为10-80μg/ml（r=0.999 3）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率为98.3%-104.9%（RSD=2.0%,n=9）。结论：该方法简便、准确、专属性强,可用于测定当归芍药散中芍药苷的含量。OBJECTIVE：To establish a method for the content determination of peoniflorin in Danggui shaoyao powder,and provide a reference for controlling the quality of the preparation. METHODS：HPLC was performed on the column of Symmetry C_（18） with mobile phase of acetonitrile-water（containing 0.1% phosphoric acid）（14 ∶ 86,V/V）at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 20 ℃,and injection volume was 20 μl. RESULTS：The linear range of peoniflorin was 10-80 μ g/ml（r=0.999 3）;RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was98.3%-104.9%（RSD=2.0%,n=9）. CONCLUSIONS：The method is simple,accurate and specific,and can be used for the content determination of peoniflorin in Danggui shaoyao powder.

关 键 词：当归芍药散 芍药苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]