等长收缩运动对动脉粥样硬化兔血管内皮生长因子与一氧化氮的影响  

Effects of isometric exercise on vascular endothelial growth factor and NO levels in atherosclerosis

作　　者：赵彦[1] 赵美丽[2] 林爱翠[1] Zhao Yah Zhao Meili Lin Aicui(Department of Sports and Health Science, Nanjing Sports Institute, Nanjing 210014, China)

机构地区：[1]南京体育学院运动健康科学系,南京210014 [2]南京医科大学附属南京医院(南京市第一医院)

出　　处：《中华物理医学与康复杂志》2016年第10期726-729,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金（81101456）;江苏省体育局课题（ST12100203）;南京市医学科技发展资金资助南京市卫生青年人才培养工程项目（QRX11248,KK14097）;南京医科大学科技发展基金项目（2013NJMU077）;江苏省高校自然科学研究面上项目（14KJB320002）

摘　　要：目的探讨等长收缩运动（IE）对动脉粥样硬化兔血管内皮生长因子（VEGF）和一氧化氮（NO）的影响。方法选取成年雄性新西兰白兔24只，制作IE训练模型后，按照随机数字表法分为对照组、高脂组和训练组进行2个阶段（6周＋4周）的实验，每组8只。对照组采用普通饮食，其余两组采用高脂饲料喂养。训练组兔在第2阶段增加每次2rain、每日3次、每周5d的IE训练。分别于实验前、实验第6周及第10周时对兔进行外周血VEGF、VEGFmRNA与NO检测，并于实验第10周时对兔主动脉血管壁进行动脉粥样硬化斑块检测。结果实验前，各组VEGF、VEGFmRNA、NO含量比较，差异均无统计学意义（P〉0．05）。10周后，高脂组兔外周血VEGF含量高于训练组和对照组，差异有统计学意义（P〈0．05）。训练组和对照组各时间点兔外周血VEGF含量比较，差异均无统计学意义（P〉0．05）。高脂组兔实验10周后外周血VEGF含量高于实验前（P〈0．05）。实验10周后，训练组和高脂组兔外周血VEGFmRNA含量均高于对照组（P〈0．05）。3组兔实验10周后外周血VEGFmRNA含量均高于实验前和实验6周后（P〈0．05）。实验6周后，训练组NO含量高于对照组（P〈0．05）。实验10周后，高脂组NO含量高于对照组（P〈0．05）。对照组、高脂组、训练组兔外周血NO含量在第6周和第10周时均高于实验前（P〈0．05）。实验结束时，训练组兔有少量动脉粥样硬化斑块形成，高脂组兔动脉粥样硬化斑块最多，对照组兔无动脉粥样硬化斑块形成。结论高脂饮食可以促进动脉粥样硬化形成，使VEGF、VEGFmRNA和NO含量显著增加，IE训练可有效减缓动脉粥样硬化进程，其具体机制尚待进一步研究。Objective To explore the effect of isometric contraction exercise （IE） on the levels of vascular endothelial growth factor （VEGF） and nitric oxide （NO） in atherosclerosis. Methods Twenty-four New Zealand rabbits were randomly divided into a control group, a high fat （HF） group and a high fat with training （FT） group, each of 8. The rabbits in the control group were fed with normal food, while those in the other 2 groups were provided with a high-fat diet. Beginning six weeks later, the FT group received 2 minutes of IE training 3 times a day, 5 days a week for 4 weeks. VEGF, VEGF mRNA and NO were examined before the study and six and ten weeks after the in- tervention began. Atherosclerotic plaques were tested at the end of the intervention. Results Before the intervention there was no significant difference in any of the measurements among the 3 groups. After the intervention the average VEGF in the HF group was significantly higher than in the other 2 groups and higher than before the intervention. However, no significant differences in average VEGF were observed between the FT and control groups at any time point. After 10 weeks the average VEGF mRNA of both the HF and FT groups was significantly higher than that of the control group. Moreover, the average VEGF mRNA in each group was significantly higher than before and after the 6-week intervention. The average NO content of the FT and HF groups was significantly higher than that of the controlgroup six and ten weeks after intervention. For all 3 groups, the measurements 6 and 10 weeks later were significantly higher than before the intervention. More atherosclerotie plaques were observed in the HF group than in the FT group, while no atherosclerotic plaques were found in the control group. Conclusion A high-fat diet can promote the for- mation of atheroselerotic plaques and the production of VEGF, VEGF mRNA and NO. IE training can effectively slow the development of atherosclerosis, but the mechanism needs further research.

关 键 词：等长收缩 动脉粥样硬化 血管内皮生长因子 一氧化氮 

分 类 号：R543.5[医药卫生—心血管疾病]