hBMP-2修饰β-TCP/Ⅰ型胶原复合材料对MC3T3-E1细胞增殖的影响  

Effect of hBMP-2 Modified beta TCP/Collagen Ⅰ Composite on the Proliferation of MC3T3-E1 Cells

作　　者：赵刚[1] 朱旭佳 丁元圣[1] 莫宏兵[2] 曾娟[1] 宋天喜 ZHAO Gang ZHU Xu- jia DING Yuan - sheng MO Hong - bing ZENG Juan SONG Tian - xi(Department of Orthodontics, The Second Affiliated Hospital of Jiamusi University, Jiamusi 154004, China Department of Prosthodontics , The Second Affiliated Hospital of Jiamusi University, J iamusi 154004, China Beijing Austrian Fine Pharmaceutical Technology Co. , Ltd. Beijing 100176, China)

机构地区：[1]佳木斯大学附属第二医院正畸科,黑龙江佳木斯154004 [2]佳木斯大学附属第二医院修复科 [3]北京奥精医药科技有限公司,北京100176

出　　处：《口腔医学研究》2016年第10期1028-1032,共5页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金(编号:H201487)

摘　　要：目的:探究含人的骨形成蛋白-2为目的基因的质粒DNA修饰纳米β-磷酸三钙(Tricalcium phosphate)/Ⅰ型胶原溶液复合材料对小鼠前成骨细胞增殖的影响。方法:含hBMP-2为目的基因的质粒DNA修饰纳米β-TCP/Ⅰ型胶原溶液复合材料,建立MC3T3-E1细胞株与纳米复合材料体外培养体系。将其分为支架组和平皿组,扫描电镜、光镜观察细胞学形态,Alamar Blue法检测细胞增殖,MuseTMCell Analyzer法检测细胞周期,实时荧光定量PCR检测Cdk2、Cdk4等细胞增殖基因。结果:复合材料细胞黏附数、增殖数及表面分布高于平皿组(P<0.05),实验结果显示支架组均优于平皿组。结论:复合材料与单纯含hBMP-2为目的基因的质粒DNA相比,更能促进前成骨细胞黏附、增殖及功能代谢,表明其对前成骨细胞具有良好细胞相容性,可成为一种新型的具有应用前途的骨修复或组织工程材料。Objective：To explore the effect of hBMP-2modified beta TCP/collagenⅠcomposite on the proliferation of MC3T3-E1 cells.Methods：Nano beta TCP/collagenⅠsolution composite materials were modified by plasmid DNA containing hBMP-2as the target gene,then the MC3T3-E1 cell line and nano composite materials in vitro culture system were established.Cells were divided into stent group and plate group,scanning electron microscope（SEM）and optical microscopy were used to observe the cell morphology,while cell proliferation and cycle were detected by Alamar Blue and MuseTM Cell analyzer assays,respectively.Real-time fluorescence quantitative PCR were applied to measure cell proliferation genes Cdk2 and Cdk4.Results：Cell adhesion,proliferation and distribution of the stent group were higher than those of the plate group（P〈0.05）.Conclusion：Compared to the plasmid DNA containing hBMP-2only,hBMP-2modified beta TCP/collagenⅠcomposite was better at stimulating preosteoblast adhesion,proliferation and metabolism.It might be used as bone repair or tissue engineering materials.

关 键 词：BMP-2 β-TCP/胶原 增殖 

分 类 号：R318.08[医药卫生—生物医学工程]