T1ρ成像技术监测淫羊藿治疗早期兔膝软骨退变  

T1ρmagnetic resonance imaging for detection of early articular cartilage degeneration in knees of rabbit model treatment by Epimedium

作　　者：张景[1] 张水花[2] 沈思[3] 刘斯润[3] ZHANG Jing ZHANG Shuihua SHEN Si LIU Sirun(Department of Radiology ,the Third Affiliated Hospital of Guangzhou Medical University ,Guangzhou 510150,China)

机构地区：[1]广州医科大学附属第三医院放射科,广东广州510150 [2]广州中医药大学附属第一医院放射科 [3]暨南大学第一附属医院影像中心

出　　处：《实用放射学杂志》2016年第10期1609-1613,共5页Journal of Practical Radiology

摘　　要：目的：探讨 T1ρ成像技术在淫羊藿治疗兔膝软骨早期退变中的监测作用,推测淫羊藿治疗骨关节炎（OA）的疗效机理。方法建立早期 OA 模型：将28只实验兔,随机分为 A、B、C 3组,A、B 组各11只为处理组,右膝关节腔内注射0.5 mL 1.6%的木瓜蛋白酶溶液,建立早期关节炎,左膝注射等量稀释液作为对照,C 组6只为空白组。初次注药3周后对 A、B、C 3组行双膝 MR 矢状位 T2 WI、3D-FS-SPGR、T1ρ序列扫描,并于 A、B 组内随机取2只行双膝病理检查,确定早期 OA 模型建立。干预模型：A 组给予淫羊藿灌胃2月,B 组给予等剂量无菌生理盐水灌胃2月,期间于0、1、2月分别对3组同时行 MR 检查。末次扫描后处死所有试验兔并取双膝股骨髁软骨做组织病理学检查。统计分析干预前后的关节软骨厚度、T1ρ值的组内及组间差异,并与组织病理学结果对照。结果（1）关节腔内注射微量木瓜蛋白酶3周后可诱导早期兔膝骨关节炎模型建立,A、B 组诱导侧 T1ρ值均高于对照侧（P 〈0.05）,软骨厚度值无明显差异（P 〉0.05）；（2）淫羊藿干预组诱导侧软骨 T1ρ值下降（P 〈0.05）,盐水干预组 T1ρ值无明显变化（P 〉0.05）。结论（1）OA 早期、进展期软骨 T1ρ值不同程度升高,升幅与软骨基质内成分比例相关；（2）病理证实单味中药淫羊藿治疗早期兔膝骨关节炎有效,推测其可能机制为干预早期 OA 发展过程中的共同通道（蛋白酶-软骨基质降解）,抑制蛋白酶形成并促进软骨基质中蛋白多糖分泌。Objective To explore the role of MRI（T1ρ）in the early osteoarthritis,curative efficacy monitoring and speculated that the mechanism of curative effect.Methods （1）（early OA model）A total of 28 New Zealand white rabbits were randomly divided into A,B,C three groups.group A（treatment）and B（control）were both for 1 1 rabbits,and 6 rabbits were considered as blank control group（group C）.0.5 mL 1.6% papain was injected into the right knee joint cavity both group A and B according to three times （1,4,7 days）.The equivalent dilution was injected into the left knee joint cavity simultaneously.3 weeks after the first injection,and T2 WI,3D-FS-SPGR,and T1ρ mapping in sagittal plain were scanned for three groups.Randomly selected two models pathology test in the group A and B,confirm early OA model is established.（2）（treat models）Then give epimedium lavage to group A two month.The equivalent sterile saline lavage to group B.A certain time （T0 = 0,T1 = 1 mon,T2 = 2 mon）after treated do MR scanned for group A and B.Analysis of the image.T1ρvalues and the cartilage thickness in bilateral femoral condyle cartilage were measured by post-processing software.The all femurs were pathological examined.T1ρvalues and the cartilage thickness were statistic analysis. Results （1）Articular cavity injection of papain can successfully establish early osteoarthritis rabbit model which pathology has been confirmed.T1ρvalues of the experimental （group A,B）were significant higher than of the control side（P〈0.05）.Cartilage thickness values of the experimental （group A,B）and the control side has no statistical significance（P 〉0.05）.（2）T1ρvalues of the Epimedium treat model （right side）were significant lower than of the pre-treatment that of the preintervention （P 〈0.05 ）.T1ρvalues of the NS treatment （left side）and the pre-treatment has no statistical significance（P〉0.05）.Conclusion （1）T1ρvalues of the early and advanced stage of OA had increased in

关 键 词：膝关节退变 关节软骨 淫羊藿 磁共振成像 T1ρ 

分 类 号：R684[医药卫生—骨科学] R445.2[医药卫生—外科学]