苦瓜总皂苷对2型糖尿病大鼠肾病组织中蛋白激酶C-α和转化生长因子β表达的影响研究  

Effects of Total Saponins of Bitter Gourd on Expression of Protein Kinase C-α and Transforming Growth Factor-β in Type 2Diabetic Nephropathy Rats

作　　者：李易[1] 田鲁[1] 王港[1] 陈伟[1,2] LI Yi TIAN Lu WANG Gang CHEN Wei(Department of Kidney, Central Hospital of Huangshi, Huangshi 435000 Medical College, Hubei Institute of Technology, Huangshi 435000)

机构地区：[1]湖北省鄂东医疗集团黄石市中心医院肾内科,湖北黄石435000 [2]湖北理工学院医学院,湖北黄石435000

出　　处：《湖北中医药大学学报》2016年第5期5-9,共5页Journal of Hubei University of Chinese Medicine

基　　金：湖北省卫生厅资助荐项目(项目编号:2013FFA016)

摘　　要：目的探讨分析苦瓜总皂苷对2型糖尿病肾病大鼠蛋白激酶C-α(PKC-α)及转化生长因子β(TGF-β)表达的影响,为苦瓜总皂苷开发和临床2型糖尿病肾病的治疗提供参考。方法雄性SD大鼠48只,随机分为正常对照组、2型糖尿病肾病模型组以及苦瓜总皂苷治疗组,大鼠左肾切除+链脲佐菌素腹腔注射建立动物模型,模型建立成功后,正常对照组和模型组给予生理盐水灌胃,苦瓜总皂苷治疗组给予苦瓜总皂苷灌胃,分别于实验第4周、第8周处死大鼠各半,测血糖、24h尿蛋白定量;取肾组织做HE染色行肾组织病理观察、RT-PCR以及Western Blot检测肾组织PKC-α和TGF-β蛋白和mRNA表达。结果苦瓜总皂苷治疗组尿蛋白和血清肌酐与模型组比较明显减少,血糖明显升高,差异有显著性(P<0.05);病理组织显示正常对照组肾小球基底膜完整,没有增生,肾脏组织结构整齐,纹理清晰,模型组肾小球基底膜增厚同时伴有一定的系膜细胞增生,苦瓜总皂苷组肾小球基底膜较完整,增生较少,炎症反应也较小;RT-PCR和Western Blot可见:与模型组比较,苦瓜总皂苷治疗组肾脏组织PKC-α和TGF-β蛋白和mRNA表达明显降低(P<0.05)。结论苦瓜总皂苷可抑制糖尿病肾病大鼠肾组织PKC-α和TGF-β的表达,进而保护肾脏,可能有一定的临床应用价值。Objective To investigate the influence of total saponins of bitter ground（ TSB） on expression of protein Kinase C- α（ PKC- α） and transforming growth factor- β（ TGF- β） in type 2 diabetic nephropathy rats,to provided some references for total saponins of Bitter Gourd. Methods 48 male SD rats were randomly divided into normal control group,type 2 diabetic nephropathy model group and TSB treatment group,left kidney resection and low- dose streptozotocin intraperitoneal injection were given to built model,control group and model group received normal saline gavage for 8 weeks,TSB group received TSB gavage. Half of the rats were executed respectively at the 4th and 8th week to measure of blood glucose,24 h urinary protein; the renal tissue of renal pathology was observed with HE staining,RT- PCR and Western Blot to detect the PKC- α and TGF- β protein and mRNA expression in kidney tissue. Results TSB treatment group＇s urine protein serum creatinine decreased compared with the same period of model group,blood glucose was significantly increased,the difference was significant（ P〈0. 05）; the pathological tissue showed the integrity of glomerular basement membrane without hyperplasia neat organizational structure,clear texture of kidney of normal control group could be seen under HE staining,while model group could be seen membrane thickening with mesangial cell proliferation. Extracellular matrix significant proliferation,the glomerular basement membrane of TSB group was complete with less hyperplasia and less inflammatory response; RT- PCR and Western Blot showed that,compared with normal group,the expression of PKC- α,TGF- β protein and mRNA in TSB group decreased significantly（ P〈0. 05）. Conclusion TSB can inhibit PKC- αand TGF- β ofrenal tissue of type 2 diabetic nephropathy rats,and protect the kidneys,it may have some clinical value.

关 键 词：糖尿病肾病 苦瓜总皂苷 蛋白激酶C-Α 转化生长因子Β 

分 类 号：R259[医药卫生—中西医结合]