杀藻菌株Deinococcus sp.Y35的培养基优化及其杀藻菌剂的制备  

Culture medium optimization for algicidal strain Y35 and preparation of algicidal bacterial agents

作　　者：李祎[1,2] 姜晓冰[2] 刘磊[2] 朱红[1] 王海磊[2] 郑伟[1] 郑天凌[1] 

机构地区：[1]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室,厦门361102 [2]河南师范大学生命科学学院,新乡453007

出　　处：《应用与环境生物学报》2016年第5期897-903,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(41576109,31500095);河南师范大学博士科研启动基金(5101049170160)资助~~

摘　　要：塔玛亚历山大藻是一种有毒甲藻,常引发赤潮,严重威胁海洋生态的稳定和人类的健康,细菌Deinococcus sp.Y35表现出对塔玛亚历山大藻的杀藻能力,为促进菌株生长、提高杀藻效果并方便保存,对菌株Y35培养条件进行优化,并制备杀藻菌剂.分别确定菌株Y35生长所需的最适氮源、碳源、无机盐,并确定其最适添加量,在优化的基础上完成冻干菌剂的制备和最适冻干保护剂的选择.菌株Y35生长的最适培养基成分是1.0%胰蛋白胨和0.5%酵母粉.在优化的培养基基础上对菌株Y35进行发酵,培养至对数期后进行冷冻干燥,制备杀藻菌剂.菌株Y35需要添加1.0 g/L的蔗糖作为冻干保护剂.杀藻菌剂的杀藻添加量为2.0 mg/m L.本研究可为下一步将细菌应用于赤潮治理奠定基础.Alexandrium tamarense is a toxic dinoflagellate,which causes harmful algal blooms（HABs）,posing a serious threat on marine ecology and human health.Deinococcus sp.Y35 shows algicidal activity on A.tamarense.This research aimed to optimize its culture medium and improve the preparation of bacterial agents.We determined the optimum nitrogen source,carbon source and inorganic salts of strain Y35,then decided the optimum adding amount,prepared the freeze drying bacterial agents,and chose the optimum freeze-drying protective agent.The optimum culture medium composition of strain Y35 was found to be 1% tryptone and 0.5% yeast extracts.We needed to add 1.0 g/L sucrose as a protective agent for bacterial culture of strain Y35 before freeze-drying.The optimum amount of algicidal agent was 2.0 mg/m L.The optimization of culture conditions could ensure the bacterial growth and algicidal activity.The results of this research provide some foundation for HABs control in the future.

关 键 词：DEINOCOCCUS sp.Y35 塔玛亚历山大藻 培养基优化 杀藻菌剂 

分 类 号：X55[环境科学与工程—环境工程]