每周单次皮下注射rhPTH(1-34)促进大鼠的骨折愈合  

Once-weekly subcutaneous injection of recombinant human parathyroid hormone (1-34) promotes the bone fracture healing in rats

作　　者：马腾[1] 张稳[1] 郭琦[1] 海宝[1] 刘灿[1] 宋纯理[1] 

机构地区：[1]北京大学第三医院骨科,北京100191

出　　处：《中国实验动物学报》2016年第5期481-486,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81171693;81100895);国家高技术研究发展计划(863计划)(No.SS2015AA020304)

摘　　要：目的观察每周单次皮下注射甲状旁腺激素rhPTH(1-34)促进大鼠骨折愈合的效果。方法 50只3月龄雄性SD大鼠左侧胫骨骨折,克氏针内固定术后,根据皮下注射rhPTH(1-34)的剂量及频次,随机分为5组(n=10/组):每周10、20μg/kg组;每日10、20μg/kg组;每周皮下注射等量(500μL)生理盐水作为对照组。治疗4周后处死取材,分别行小动物X线、双能X线、micro-CT及三点弯曲生物力学实验检测骨折愈合情况。结果无论每周或每日皮下注射rhPTH(1-34)骨密度均高于对照组(P<0.05);每周20μg/kg组骨折愈合效果好于对照组,其中rhPTH(1-34)每周20μg/kg组在骨密度、矿化的骨痂体积、骨痂总体积、矿化的骨痂体积占骨痂总体积百分数、最大载荷方面分别比对照组高26.2%、51.4%、21.6%、24.5%、29.3%。每周20μg/kg、每日10μg/kg及每日20μg/kg组的最大载荷均高于对照组(P<0.01);每周20μg/kg组骨密度、骨微结构、力学强度均与每日10μg/kg组无统计学差异(P>0.05)。结论每周皮下注射甲状旁腺激素(rhPTH1-34)(10、20μg/kg)可以促进骨折愈合,但相同单次剂量,每日皮下注射的效果仍优于每周皮下效果。Objective To study the effect of once-weekly injection of recombinant human parathyroid hormone rh PTH（ 1-34） on the healing of bone fracture in rats. Methods Fifty male 3-month old SD rats were used in this study to produce unilateral tibial fracture and received internal fixation with a Kirschner needle. Based on the dose and frequency of rh PTH（ 1-34） injection,the rats were randomly divided into five groups（ n = 10 each） as follows： subcutaneous injections of saline,and rh PTH in a dose of 10,20,10,and 20 μg/kg/d. After 4 weeks of treatment,the rats were euthanatized and the fractured tibia were assessed by X-ray,dual energy X-ray absorptiometry（ DXA）,micro-computed tomography（ micro CT） and three-point bending test. Results The fracture healing in the 20 μg/kg/w group was better than the saline group. The fracture healing in the 20 μg/kg/w group was as well as the 10 μg/kg/dgroup. The BMD of 20 μg/kg/w group was 26. 2% higher than the saline group. The mineralized callus volume in the 20 μg/kg/w group was 51. 4% higher than the saline group. The total callus volume in the 20 μg/kg/w group was 21. 6% higher than the saline group. The ultimate load of the 20 μg/kg/w group was 29. 3% higher than the saline group. There was no significant difference between the20 μg/kg/w group and 10 μg/kg/d group in BMD,bone micro-architecture,and biomechanical strength（ P〉0. 05）.Conclusions Once weekly injection of rh PTH（ 1-34） can promote the bone fracture healing.

关 键 词：甲状旁腺激素片段 骨折愈合 每周给药 每日给药 大鼠 

分 类 号：Q95-33[生物学—动物学]