裸鼠肺癌脑转移模型中血脑屏障超微结构的观察  

Ultrastructural observation of blood-brain barrier in the nude mouse model of brain metastases from lung cancer

作　　者：陈愉生[1] 涂洵崴 俞梅娥 陈正伟[1] 李鸿茹[1] 钟秀容[1] 周琳瑛[1] 

机构地区：[1]福建医科大学省立临床医学院,福州350001

出　　处：《中国实验动物学报》2016年第5期494-498,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家卫生和计划生育委员会科研基金(WKJ-FJ-17;2013-2016);福建省立医院优秀青年医师项目(2014YNQN03);福建省自然科学基金(2015J01376)

摘　　要：目的通过硝酸镧示踪电镜技术观察裸鼠肺癌脑转移模型中血脑屏障的超微结构。方法从8只BALB/c裸鼠随机选取6只经左心室注射的方法,接种处于对数期生长的人肺腺癌细胞PC-9(1×106/0.1 m L),作为模型组;另2只裸鼠未经任何处理作为空白组。接种后观察裸鼠状态,在第4周行硝酸镧示踪电镜技术观察血脑屏障的超微结构,并留取肺、脑脏器,病理HE染色观察。结果模型组裸鼠于第3周开始出现体重减轻,并逐渐出现恶液质,全部裸鼠于第4周处死。肉眼取材时,胸廓、肺、脑、脏器均未见到转移灶。HE染色示:模型组裸鼠均出现大小不一的多发脑转移灶。硝酸镧示踪电镜示,模型组可见硝酸镧颗粒侵入到血管腔外,稀疏或者弥漫地分布在脑组织中,空白组镧颗粒仅沉积在血管腔内。结论弥散分布地镧颗粒说明了模型中血脑屏障结构的不完整性,而肺癌脑转移的发生伴随着血脑屏障结构的破坏。Objective To observe the ultrastructure of blood-brain barrier in the nude mouse model of brain metastases from lung cancer by transmission electron microscopy using lanthanum nitrate tracing. Methods PC-9 cells（ 1 ×106/0. 1 m L） in logarithmic phase were respectively injected into six nude mice（ model group） selected from eight nude mice randomly via the left ventricle,the other two mice without any treatment as the control group. The general status of the mice was observed after implantation. In the fourth week all the mice were sacrificed and brain tissue samples were taken and prepared for transmission electron microscopic observation using lanthanum nitrate tracing. besides,the lung and brain were removed and stained with HE to detect the presence of tumor metastasis. Results Mice in the model group began to lose weight almost simultaneously in the third week and became moribund slowly,and were all sacrificed at the fourth week when showing clear signs of cachexia. At autopsy,the thoraxes were clear,with normal lungs. Histology showed evidence of brain metastasis in all the six mice. The electron microscopy showed that lathanum nitrate tracer was escaped from the capillaries and diffusely or sparsely distributed in the brain tissues of the model group mice,however lathanum nitrate tracer was still confined in the capillary lumen in the mice of control group. Conclusions The diffuse lathanum nitrate tracer in the brain parenchymal tissue indicates the impairment of blood-brain barrier in the nude mouse model of lung cancer brain metastasis and the formation of these metastases is accompanied with the destruction of blood brain barrier.

关 键 词：肺癌脑转移 血脑屏障 硝酸镧 透射电镜 左心室注射 裸鼠 

分 类 号：Q95-33[生物学—动物学]