文蛤肉水解液的致突变性  

Mutagenicity evaluation of the hydrolysate of Meretrix meretrix Linnaeus soft tissue

作　　者：高梅 曹冲 王功霞 汤连升 贾庆文 

机构地区：[1]山东省药学科学院,山东省化学药物重点实验室,济南250101

出　　处：《中国实验动物学报》2016年第5期521-525,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：山东省自主创新及成果转化专项(项目编号:2014ZZCX02104)

摘　　要：目的评价文蛤肉水解液的致突变性,为文蛤的开发利用提供实验数据。方法采用Ames试验、小鼠嗜多染红细胞骨髓微核试验和CHL细胞体外染色体畸变试验3项致突变性试验,检测文蛤肉水解液有无致突变作用。结果 Ames试验中文蛤肉水解液各剂量组回变菌落数均在正常范围内,均未超过自发回变菌落数的2倍,在加与不加S9时5株试验菌株结果为阴性。CHL细胞体外染色体畸变试验,文蛤肉水解液各剂量组的染色体畸变率与阴性对照组比较,差异无显著性(P>0.05),结果为阴性。小鼠骨髓微核试验,各剂量组的微核率与阴性对照组比较,差异无显著性(P>0.05),结果为阴性。结论在本试验条件和范围下,文蛤肉水解液未见潜在的致突变作用。Objective To evaluate the mutagenicity of hydrolysate of Meretrix meretrix Linnaeus soft tissue,so as to provide experimental basis for its exploitation. Methods Three mutagenicity tests were used to evaluate the mutagenic effects,including Ames test,CHL chromosome aberration assay and bone marrow micronucleus assay in mice. Results In Ames test,the revertant colonies numbers in each group were twice less than the numbers of spontaneous revertant colonies,five bacterial strains showed negative results with or without S9 activation,and the result of Ames test was negative.The CHL chromosome aberration assay and bone marrow micronucleus assay showed that the chromosome aberration rate and micronucleus rate of each dose group showed no significant difference compared with the negative control group,respectively（ P〉0. 05）. Conclusions Under this condition,the results show that all of the Ames test,chromosome aberration assay and bone marrow micronucleus assay are negative,and no mutagenicity is observed in the hydrolysate of Meretrix meretrix Linnaeus soft tissue.

关 键 词：文蛤 致突变性 AMES试验 染色体畸变试验 微核试验 

分 类 号：Q95-33[生物学—动物学]