鹿特异性复合麻醉剂对大鼠脑区NOS活性及NO/cGMP信号转导系统的影响  

Effects of the Specificity Anesthetic for Deer on NOS activity and NO/cGMP Signal Pathway in Different Encephalic Region of Rats

作　　者：尹柏双[1] 宋永利[1] 付连军[1] 呼显生[1] 沙万里[1] 方文山[1] 高利[2] 

机构地区：[1]吉林农业科技学院动物医学学院,吉林吉林132101 [2]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2016年第9期114-116,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(31302150);长白山动植物资源保护与利用省级重点实验室基金项目(吉农院合字2012701;2013S029);吉林农业科技学院博士启动基金项目(吉农院合字2012307);吉林农业科技学院种子基金项目(吉农院合字2013905);吉林省教育厅"十二五"科学技术研究(吉教科合字2014379)

摘　　要：研究鹿特异性复合麻醉剂麻醉下大鼠不同脑区NOS活性、NO和c GMP浓度的变化,探讨鹿特异性复合麻醉剂的中枢作用机理。将24只SD大鼠随机分成4组,分别为对照组、诱导期、麻醉期和催醒期,于不同时期采集大鼠大脑皮质、小脑、脑干、海马和丘脑。采用比色法测定各脑区NOS活性和NO含量,酶联免疫吸附法测定各脑区c GMP浓度。结果表明,腹腔注射鹿特异性复合麻醉剂30 mg/kg体重后,麻醉期各脑区一氧化氮合酶(NOS)活性显著降低(P<0.05或P<0.01);NO产量与对照组比较降低极显著(P<0.01);鸟甘酸环化酶(c GMP)浓度降低显著(P<0.05或P<0.01)。结果提示,鹿特异性复合麻醉剂抑制大鼠各脑区NOS活性,阻断NO/c GMP信号转导可能是其产生全麻作用的重要机理之一。NOS activity,NO and c GMP contents in different encephalic regions of rats were measured to explore the possible molecular mechanism of action of the deer specific anesthetic on the central nervous system.24 SD rats were divided randomly into four groups including the control,the induction the anesthesia and the recovery.Rats＇ brain samples of cerebral cortex,hippocampus,cerebellum,thalamus and stem were separated at the different time points under the deer specific anesthesia.The activities of NOS were measured by using colorimetry,and the concentrations of NO and c GMP were determined by Elisa. The NOS activities（P〈0.05 or P〈0.01）,the NO contents（P〈0.01）,and the concentration of c GMP（P〈0.05 or P〈0.01）were all obviously decreased in all encephalic regions in the anesthesia group compared with control groups.These results indicated that the deer specific anesthetic inhibited NOS activity and blocked NO/c GMP signal transduction,which might be one of the important mechanisms for the drug to generate general anesthesia.

关 键 词：鹿特异性复合麻醉剂 一氧化氮合酶 一氧化氮/环鸟苷酸 信号转导 

分 类 号：S859.791[农业科学—临床兽医学]