北京中国农大小型猪三个亚系群体的遗传状况分析  

Population genetic quality analysis of 3 subbreeds of China Agricultural University miniature pigs in Beijing

作　　者：魏杰[1] 巩薇[1] 王洪[1] 李晓波[1] 付瑞[1] 王吉[1] 邢进[1] 冯育芳[1] 王淑菁[1] 高正琴[1] 岳秉飞[1] WEI Jie Gong Wei Wang Hong LI Xiao-bo FU Rui Wang Ji XING Jin FENG Yu-fang WANG Shu-jing GAO Zheng-qin YUE Bing-fei(National Institute for Food and Drug Control, Beijing 100050, China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国比较医学杂志》2016年第10期50-55,共6页Chinese Journal of Comparative Medicine

基　　金：中国食品药品检定研究院中青年基金(2014C4)

摘　　要：目的对北京地区中国农大小型猪三个亚系群体进行遗传质量评价与分析。方法利用DB11/T828.3-2011中的25对微卫星引物,对3个中国农大小型猪亚系群体进行微卫星分型,利用群体遗传分析软件Popgen32对所得结果进行处理分析。结果农大I系、农大II系和农大III系小型猪群体分别得到130、122和138个等位基因,平均杂合度分别为0.6759、0.5967、0.6779,平均多态信息含量(PIC)分别为0.6344、0.5540、0.6403;农大II系和农大III系的遗传距离为0.4251,农大I系与农大II系的遗传距离为0.2084。结论三个亚系中,农大II系和农大III系小型猪均具有较好的遗传多样性和稳定性,符合封闭群动物的群体遗传特征。Objective To analyze and evaluate the population genetic quality of 3 subbreeds of China Agricultural University miniature pigs in Beijing. Method According to the local standard DB11 / T828. 3- 2011,25 pairs of microsatellite primers were used in 3 subbreeds of China Agricultural University miniature pigs,and software Popgen32 was used to process the data. Results 24 microsatellite loci shared 130,122 and 138 alleles in the China Agricultural University miniature pigs I, II, III, respectively. The average heterozygosity was 0. 6759, 0. 5967 and 0. 6779,respectively,while the average polymorphism information content（ PIC） was 0. 6344,0. 5540 and 0. 6403,respectively.The genetic distance between China Agricultural University miniature pig II and III was 0. 4251,while the genetic distance between China Agricultural University miniature pig I and II was 0. 2084. Conclusions In the 3 subbreeds,China Agricultural University miniature pigs II and III have genetic stability and genetic diversity,and both of which satisfy with the genetic characteristics of closed colony laboratory animal.

关 键 词：农大小型猪 微卫星标记 亚系 群体遗传 

分 类 号：R33[医药卫生—人体生理学]