缬沙坦对急性心肌梗死大鼠血管新生及miR29表达的影响  

Effect of valsartan on angiogenesis and miR29 expression in rats with acute myocardial infarction

作　　者：宋恒良[1] 胡黎坤 

机构地区：[1]郑州大学第二附属医院心内科,450014 [2]河南省南阳市油田总医院

出　　处：《中国实用医刊》2016年第20期19-21,F0004,共4页Chinese Journal of Practical Medicine

摘　　要：目的观察缬沙坦对心肌梗死后血管新生及miR29表达的影响。方法将60只Wistar雄性大鼠按随机数字法分为模型组、假手术组、缬沙坦组。结扎大鼠左前降支动脉建立心肌梗死模型，缬沙坦组术后给予缬沙坦30mg／（kg·d）灌胃4周，模型组及假手术组给予等量助溶溶剂灌胃。4周后处死大鼠，取前壁心肌梗死组织，免疫组化染色方法检测微血管密度，检测miR29表达水平。结果缬沙坦组微血管计数较模型组增加，模型组较假手术组增加，差异有统计学意义（P〈0．05）；缬沙坦组miR29较其他两组表达水平下降（P〈0．01），模型组和假手术组间比较差异有统计学意义（P〈0．01）。结论缬沙坦可以促进急性心肌梗死大鼠血管新生，可以降低miR29的表达，抑制急性心肌梗死后大鼠的心室重构。Objective To observe the effect of valsartan on angiogenesis and miR2,9 expression after myocardial infarction. Methods Sixty male Wistar rats were randomly divided into model group, sham operation group and valsartan group. Left anterior descending artery was Ligated to establish myocardial infarction model. After operation, valsartan group received valsartan 30 mg/（ kg· d） by gavage for 4 weeks, the model group and sham operation group were given equivalent solvent orally. After 4 weeks, the animals were sacrificed and the expression of miR29 was detected by immunohis to chemical staining of myocardium in the infarct area. Results In the valsartan group, micro vessel count compared with the model group increased, model group compared with the sham operation group increased, the differences were significant （ P 〈 0. 05 ）. The expression of miR29 levels in valsartan group decreased （ P 〈 0. 01 ）, there were significant differences between model group and sham operation group （P 〈 0.01 ）. Conclusions Valsartan can inhibit ventricular remodeling in rats after acute myocardial infarction. It can promote myocardial angiogenesis in rats with acute myocardial infarction and reduce the expression of miR29.

关 键 词：心肌梗死 心肌重构 血管新生 miR29 

分 类 号：R542.22[医药卫生—心血管疾病]