极化液对内毒素血症小鼠脾脏的保护作用  

Roles of GIK on spleen in a mouse model of endotoxemia

作　　者：惠永岗 杨宇焦 陈昌林[2] 余庆波[2] 马丁 万勇[2] 

机构地区：[1]咸阳市中心医院麻醉科,陕西咸阳712000 [2]川北医学院附属医院麻醉科,四川南充637000

出　　处：《川北医学院学报》2016年第5期659-662,共4页Journal of North Sichuan Medical College

基　　金：四川省教育厅重点项目(16ZA0238)

摘　　要：目的:探讨高浓度极化液(GIK)对脂多糖(lipopolysaccharide,LPS)诱导内毒素血症小鼠脾脏损伤的保护作用。方法:连续3 d腹腔注射脂多糖(LPS)5 mg·kg-1·d-1,构建内毒素血症小鼠模型,运用ELISA、脾脏指数、组织病理学等方法明确GIK对内毒素血症小鼠脾脏损伤是否起保护作用。结果:与LPS组小鼠比较,GIK组小鼠脾脏中促炎细胞因子TNF-α、IL-1β表达显著下调(P<0.01),抑炎因子IL-10表达上调(P<0.01);脾脏指数显著升高(P<0.05);脾脏损伤明显减轻。结论:GIK对内毒素血症小鼠脾脏损伤具有保护作用。Objective： To explore the protection effect of high concentration polarization（ GIK） on endotoxemia mice spleen injury induced by lipopolysaccharide. Methods： Endotoxemia mice model induced by 5 mg·kg- 1·d- 1lipopolysaccharide injection intraperitoneally for 3 consecutive days. To apply ELISA,splenic index,histopathology to make sure if there was a protection effect of GIK on endotoxemia mice spleen injury. Results： Compared with lipopolysaccharide group,the express of pro-inflammatory cytokines TNF-α、IL-1β of GIK group was down-regulated obviously（ P〈0. 01）,anti-inflammatory cytokine IL-10 was up-regulated（ P〈0. 01）; the spleen index was increased notably（ P〈0. 05）; the spleen injury was attenuated significantly. Conclusion： GIK has a protection effect on endotoxemia mice spleen injury.

关 键 词：脂多糖 内毒素血症 脾脏损伤 极化液 

分 类 号：R631[医药卫生—外科学]