Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon  

作　　者：Gaojie Ji Jie Zhang Haiying Zhang Honghe Sun Guoyi Gong Jianting Shi Shouwei Tian Shaogui Guo Yi Ren Huolin Shen Junping Gao Yong Xu 

机构地区：[1]Beijing Vegetable Research Center,National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China [2]Department of Vegetable Science College of Horticulture China Agricultural University Beijing 100097,China

出　　处：《Journal of Integrative Plant Biology》2016年第9期762-765,共4页植物学报（英文版）

基　　金：supported by grants from National Natural Science Foundation (31361140355, 31401893, 31272184);National Key Development Plan Pilot Projects (Functional genomics research and application of wheat and other crops);Beijing Scholar Program and Beijing Excellent Talents Program (2014000021223TD03);Beijing Municipal Science & Technology Commission (6141001, 6144023, WRDMC01);Ministry of Science and Technology of China (2015BAD02B0202, 2014BAD01B08);Ministry of Agriculture of China (CARS-26)

摘　　要：Summary Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate syn- thase 4 CCitACS4）, expressed specifically in carpel primor- dia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism （SNPs） and one lnDel identified in the coding region of CitACS4, the C364W mutation located in the conserved box 6 was co- segregated with andromonoecy. Enzymatic analyses showed that the C364W mutation caused a reduced activity in CitACS4. We believe that the reduced CitACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers.Summary Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate syn- thase 4 CCitACS4）, expressed specifically in carpel primor- dia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism （SNPs） and one lnDel identified in the coding region of CitACS4, the C364W mutation located in the conserved box 6 was co- segregated with andromonoecy. Enzymatic analyses showed that the C364W mutation caused a reduced activity in CitACS4. We believe that the reduced CitACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers.

关 键 词：1-aminocyclopropane-1-carboxylic acid synthase Citrullus lanatus sex determination 

分 类 号：S651[农业科学—果树学]