Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination  

作　　者：Bingxian Chen Jun Ma Zhenjiang Xu Xiaofeng Wang 

机构地区：[1]Seed Science and Technology Lab,College of Life Sciences,South China Agricultural University [2]Agro-biological Gene Research Center,Guangdong Academy of Agricultural Sciences

出　　处：《Journal of Integrative Plant Biology》2016年第10期859-869,共11页植物学报（英文版）

基　　金：funded by a grant (no.30870224) from the Natural Science Foundation of China to XW

摘　　要：The purpose of this study was to investigate the role of cellulase in endosperm cap weakening and radicle elongation during lettuce （Lactuco sotiva L.） seed germination. The application of abscisic acid （ABA） or ethephon inhibits or promotes germination, respectively, by affecting endosperm cap weakening and radicle elongation. Cellulase activities, and related protein and transcript abundances of two lettuce cellulase genes, LsCEL1 and LsCEL2, increase in the endosperm cap and radicle prior to radicle protrusion following imbibition in water, ABA or ethephon reduce or elevate, respectively, cellulase activity, and related protein and transcript abundan- ces in the endosperm cap. Taken together, these observations suggest that celiulase plays a role in endosperm cap weakening and radicle elongation during lettuce seed germination, and that the regulation of cellulase in the endosperm cap by ABA and ethephon play a role in endosperm cap weakening. However, the influence of ABA and ethephon on radicle elongation may not be through their effects on cellulase.The purpose of this study was to investigate the role of cellulase in endosperm cap weakening and radicle elongation during lettuce （Lactuco sotiva L.） seed germination. The application of abscisic acid （ABA） or ethephon inhibits or promotes germination, respectively, by affecting endosperm cap weakening and radicle elongation. Cellulase activities, and related protein and transcript abundances of two lettuce cellulase genes, LsCEL1 and LsCEL2, increase in the endosperm cap and radicle prior to radicle protrusion following imbibition in water, ABA or ethephon reduce or elevate, respectively, cellulase activity, and related protein and transcript abundan- ces in the endosperm cap. Taken together, these observations suggest that celiulase plays a role in endosperm cap weakening and radicle elongation during lettuce seed germination, and that the regulation of cellulase in the endosperm cap by ABA and ethephon play a role in endosperm cap weakening. However, the influence of ABA and ethephon on radicle elongation may not be through their effects on cellulase.

关 键 词：Abscisic acid CELLULASE ETHEPHON endosperm cap weakening lettuce seed germination 

分 类 号：S636.2[农业科学—蔬菜学]