趋化因子CXCL1在肥胖小鼠脂肪组织中的表达及意义  

Expression and significance of chemokine CXCL1 in the adipose tissue of mice with obesity

作　　者：马斐斐[1] 曹丁丁 李伟[3] 唐认桥[3] 欧阳胜荣[1] 吴建新[1] 

机构地区：[1]首都儿科研究所生化研究室,北京100020 [2]北京大学首都儿科研究所教学医院,北京100020 [3]北京协和医学院研究生院,北京100730

出　　处：《新乡医学院学报》2016年第10期849-851,共3页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:81400365)

摘　　要：目的探讨趋化因子CXCL1在肥胖小鼠脂肪组织中的表达及意义。方法将20只小鼠随机分为高脂饲料喂养组和普通饲料喂养组,每组10只,分别给予高脂饲料和普通饲料喂养。提取2组小鼠附睾脂肪组织总RNA,应用实时荧光定量聚合酶链反应检测小鼠附睾脂肪组织中CXCL1 mRNA表达水平;成脂诱导脂肪间充质干细胞,酶联免疫吸附试验检测细胞培养上清液中CXCL1的表达。结果与普通饲料喂养组小鼠比较,高脂饲料喂养组小鼠附睾脂肪组织中CXCL1 mRNA表达显著增加(P=0.001);CXCL1蛋白表达水平随脂肪细胞分化时间延长而逐渐增加。结论小鼠肥胖状态下CXCL1表达明显增加,推测CXCL1在肥胖的发生发展过程中发挥重要作用。Objective To observed the expression and significance of chemokine CXCL1 in the adipose tissue of mice with obesity. Methods Twenty mice were randomly divided into high fat feeding group and common feeding group,10 mice in each group. The mice in high fat feeding group and common feeding group were fed with high fat diet and common diet respectively. The total RNA was extracted from the epididymal adipose tissue of mice in the two groups. The expression of CXCL1 mRNA in the epididymal adipose tissue of the mice in the two group were detected by real-time fluorescence quantitative polymerase chain reaction. The levels of CXCL1 in the cell culture supernatant was detected by enzyme-linked immunosorbent assay after adipogenic treatment of adipose-derived mesenchymal stem cells. Results Compared with common feeding group,CXCL1 mRNA expression in epididymal adipose tissue of the mice increased significantly in high fat feeding group（ P =0. 001）. CXCL1 expression increased gradually with the extending of the adipocyte differentiation time. Conclusion The expression of CXCL1 is significantly increased in the obese mice,which implys that CXCL1 plays an important role in the development of obesity.

关 键 词：肥胖 脂肪细胞分化 炎症 趋化因子 

分 类 号：R725.8[医药卫生—儿科] R723.14[医药卫生—临床医学]