黄芪注射液预处理对大鼠急性心肌缺血再灌注损伤的保护作用  

Protective effect of Astragalus injection preconditioning on acute myocardial ischemia-reperfusion injury in rats

作　　者：高军华[1,2] 千智斌[1] 

机构地区：[1]新乡医学院机能学实验室,河南新乡453003 [2]河南理工大学医院,河南焦作454000

出　　处：《新乡医学院学报》2016年第10期856-859,共4页Journal of Xinxiang Medical University

基　　金：河南省科技厅重点攻关项目(编号:102102310156)

摘　　要：目的研究黄芪注射液对急性心肌缺血再灌注损伤(MIRI)的保护作用。方法 30只成年Sprague Dawley大鼠随机分为黄芪组、缺血再灌注(I/R)组和对照组。黄芪组大鼠腹腔注射黄芪注射液40 mg·kg^(-1),每日1次,连续6 d,第7天复制大鼠左冠状动脉前降支I/R模型;I/R组大鼠腹腔注射等量生理盐水,其余实验操作同黄芪组;对照组大鼠腹腔注射等量生理盐水,不复制I/R模型。记录各组大鼠结扎前、结扎30 min、再灌注120 min及再灌注180 minⅡ导联心电图;透射电子显微镜观察大鼠心肌细胞的超微结构;反转录-聚合酶链反应检测大鼠心肌组织Na^+-K^+-三磷腺苷(ATP)酶α1亚型mRNA表达。结果与对照组比较,黄芪组和I/R组大鼠心电图ST段在结扎30 min、缺血再灌注120 min、缺血再灌注180 min时均出现缺血性改变。黄芪组大鼠心电图ST值在各时间点均显著低于I/R组(P<0.05)。黄芪组和I/R组大鼠心肌超微结构均受损,黄芪组大鼠心肌超微结构受损程度轻于I/R组;黄芪组大鼠心肌Na^+-K^+-ATP酶α1亚型mRNA表达水平显著低于对照组(P<0.01),但高于I/R组(P<0.05)。结论黄芪注射液预处理可以减轻大鼠急性MIRI,保护大鼠心功能。Objective To study the protective effect of Astragalus injection preconditioning on the acute myocardial ischemia-reperfusion injury（ MIRI） in rats. Methods Thirty adult Sprague Dawley rats were radomly divided into Astragalus group,ischemia-reperfusion（ I / R） group and control group. The rats in Astragalus group were given Astragalus injection40 mg·kg^-1·d^-1by intraperitoneal injection for 6 days; and the rats in I / R group and control group were given normal saline 40 mg·kg^-1·d^-1by intraperitoneal injection for 6 days. On the 7thday,the acute MIRI models of rats in Astragalus group and MIRI group were made by ligaturing and revascularizing the left anterior descending artery. There was no operation for the rats in control group. The Ⅱ leads electrocardiogram（ ECG） of the rats were recorded before ischemia and ischemia for30,120,180 min. The ultrastructure of cardiac muscle were observed by transmission electron microscope. The expression of Na^＋-K^＋-adenosine triphosphatase（ ATP） ase-α1 mRNA was detected by reverse transcription-polymerase chain reaction. Results Compared with the control group,the ST in ECG of rats in Astragalus group and MIRI group showed ischemic changes at the time points of the ligation for 30 minutes,ischemia-reperfusion for 120 and 180 minutes. The ST value of rats in Astragalus group was significantly lower than that in MIRI group at the each time point（ P〈0. 05）. The ultrastructure of cardiac muscle of rats in Astragalus group and MIRI group had injury,which in Astragalus group were better than that in MIRI group. The mRNA level of Na^＋-K^＋-ATPase gene-α1 subunit in Astragalus group was significantly lower than that in control group（ P 〈0. 01）,which was significantly higher than that in MIRI group（ P〈0. 05）. Conclusion Astragalus injection preconditioning can alleviate acute MIRI and protect cardiac function of rats.

关 键 词：黄芪注射液 预处理 急性心肌缺血再灌注损伤 大鼠 

分 类 号：R972[医药卫生—药品]