以DNA为靶标的芝麻油定量PCR检测技术  

THE QUANTITATIVE PCR DETECITION METHOD OF THE SESAME OIL USING DNA AS A TARGET

作　　者：王贝贝[1] 祁飞翔 李金河[1] 陈士华[1] 刘素华[1] 吴兴泉[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2016年第5期15-18,25,共5页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家科技支撑计划项目(2013BAD17B00;2013BAD17B03)

摘　　要：根据GenBank中芝麻种属特异性基因序列,利用分子生物学软件oligo7.60在其保守区设计并合成相应的特异性引物,提取芝麻DNA作为标准品模板,采用荧光定量PCR建立了芝麻油的定量检测技术。结果表明:芝麻特异性引物S199对芝麻DNA的定量检测效果最好。在芝麻油中以不同比例掺入其他食用油(大豆油),利用qPCR进行定量分析,最低检出限为1%。PCR technology plays vital roles in the false edible oil detection process by targeting on DNA of plants. The DNA is only associated with the type of plants regardless of effects of areas and climates. The aim of this study was to establish a qPCR method to detect the sesame oil with SYBR Green fluorescence. Based on the GenBank accession number of sesame $2S gene, the conserved regions were selected and the corresponding sesame-specific pairs of primers were designed by the oligo7.60.The sesame seeds genomic DNA was extracted as templates, and the optimal annealing temperature was investigated using primers $2S 1, S 176, S 199, $200 and S107. Then standard curve and sensitivity, specificity and reproducibility assay were established. Results showed that the sesame-specific pair of primer S199 was optimal in quantitative determination of sesame oil. The lowest detection rate of sesame oil DNA was 1% by mixing sesame oil with soybean oil at different ratio for qPCR quantitative analysis.

关 键 词：芝麻油 定量PCR 定量检测 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]