雷沙吉兰对MPTP诱发急性帕金森病小鼠模型的神经保护和行为学改善作用  

Effect of rasagiline on neuroprotection and behavior improvement in MPTP-induced acute mouse model of Parkinson's disease

作　　者：颜瑞佐 郭宝剑[1] 于沛[1] 张在军[1] YAN Ruizuo GUO Baojian YU Pei ZHANG Zaijun(Institute of New Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China)

机构地区：[1]暨南大学药学院新药研究所,广州510632

出　　处：《中国药科大学学报》2016年第5期603-608,共6页Journal of China Pharmaceutical University

基　　金：国家"重大新药创制"科技重大专项资助项目(No.2010ZX09103-101-055)~~

摘　　要：通过免疫组化方法检测黑质酪氨酸羟化酶阳性神经元的变化,HPLC-ECD检测纹状体内多巴胺(DA)及其代谢产物含量变化,Western blot检测黑质酪氨酸羟化酶的表达,探讨雷沙吉兰对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱发C57BL/6小鼠帕金森病模型的疗效和行为学影响。结果显示,腹腔注射MPTP的小鼠出现一系列的急性行为学改变,雷沙吉兰(20 mg/kg)治疗后黑质致密部酪氨酸羟化酶阳性细胞数目较模型组显著增加,纹状体内DA及DA代谢产物HVA和DOPAC含量也明显升高,雷沙吉兰明显改善MPTP诱导帕金森小鼠的运动行为学障碍,对帕金森小鼠模型的多巴胺系统具有保护作用。This study aimed at investigating the neuroprotective effect and behavior improvement of rasagiline on 1-methyl-4-phenyl-1,2, 3, 6-tetrahydropyridine （MPTP） model mice of Parkinson＇s disease. The tyrosine hydroxy- lase （TH） -positive dopaminergic neurons in substantia nigra were observed by immunocytochemistry. HPLC-ECD was used to detect the dopamine and its metabolite levels. Western blot was used to examine the protein expres- sion of TH. The results showed that the mice appeared a series of acute behavior change after the injection of MPTP. Rasagiline （20 mg/kg） exerted significant protection against MPTP-induced loss of TH-positive dopamin- ly. In conclusion, rasagiline has protective effect on the acute mouse model of MPTP-induced Parkinsonism.

关 键 词：雷沙吉兰 帕金森病 1-甲基4-苯基-1 2 3 6-四氢吡啶 神经保护 行为改善 

分 类 号：R965[医药卫生—药理学]