新疆产桑属植物HPLC指纹图谱研究  

Study on Fingerprints of Morus alba from Xinjiang by HPLC

作　　者：杨璐[1] 范少丽[1] 程平[1] 李宏[1] 

机构地区：[1]新疆林业科学院,新疆乌鲁木齐830054

出　　处：《安徽农业科学》2016年第26期111-114,共4页Journal of Anhui Agricultural Sciences

基　　金：自治区公益性科研院所基本科研业务经费资助项目(KY201521)

摘　　要：[目的]采用HPLC建立新疆产桑属植物指纹图谱。[方法]采用Sun Fire C18(4.6 mm×150.0 mm,5.0μm)色谱柱;以甲醇-0.2%磷酸为流动相进行梯度洗脱,检测波长330 nm,流速0.8 m L/min,柱温25℃,进样体积10μL。[结果]对13批新疆不同来源的桑葚、桑叶进行检测,结果表明,建立的桑葚、桑叶指纹图谱精密度、稳定性和重复性良好。[结论]桑葚、桑叶HPLC指纹图谱可作为新疆桑属植物鉴别与质量控制的科学依据。[Objective] To establish the fingerprints of Morus alba.from Xinjiang by HPLC.[Methods] The samples were separated on SunFire C18 （4.6 mm ×150.0 mm,5.0 μm） with methanol-0.2% phosphoric acid as the mobile phase to conduct gradient eluent at the flow rate of 0.8 mL/min at 30℃,and the detection wavelength was 330 nm.The sample injection was 10μL.[Results] 13 samples of mulberry and mulberry leaves from different origins of Xinjiang were detected.The results showed that the fingerprints had good accuracy,repeatability and stability. [Conclusion] The HPLC fingerprints can be used as the scientific evidence for identification and quality control of M.alba from Xinjiang.

关 键 词：新疆 桑属植物 指纹图谱 高效液相色谱 

分 类 号：S188[农业科学—农业基础科学]