丹参植株内生菌的分离纯化及其抗病性研究  

Isolation and Anti-microbial Activity of Endophytes fromSalvia miltiorrhiza

作　　者：颜华[1] 何姣[1] 李素俭[1] 贾良辉[1] 李军超[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2016年第9期1813-1818,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31101476);2012年度高等学校博士学科点专项科研基金(20120204120042);西北农林科技大学引进人才专项(Z111021104);杨凌示范区科技计划项目(2014NY-41)

摘　　要：为探明丹参(Salvia miltiorrhiza Bge)内生菌的种类,筛选拮抗农作物病害菌的生防菌株,从健康丹参植株中进行内生菌分离,依照形态特征以及16SrDNA序列对菌株进行初步分类鉴定,采用琼脂块法进行拮抗菌株筛选,并选取拮抗活性较强的菌株液体发酵进行体外抑菌试验。结果表明:(1)从丹参植株中共分离得到69株内生菌(真菌62株、放线菌7株),其中23株内生真菌属于无孢类群或现有条件不适合产孢,其余真菌菌株中串珠镰孢菌属(Fusarium moniliforme Sheld.)和交链孢菌属(Alternaria sp)为优势菌群;7株内生放线菌均为链霉菌属。(2)病原菌拮抗性实验表明,有44株内生菌对病原菌具有不同程度的拮抗活性,其中12株内生菌对2种及以上靶标病原菌具有拮抗活性,表明丹参内生菌具有一定的广谱抗菌性。(3)放线菌菌株A232的次级代谢产物对白色假丝酵母(Canidia albicans)和苹果腐烂菌(Valsa mali)都表现出较强的拮抗活性,通过形态、培养特征以及16SrDNA序列分析,鉴定其为Streptomyces luteoverticillatus。The objective of this research was to explore the population diversity,distribution features and the antibacterial activity of endophytes of Salvia miltiorrhiza Bge.69 strains were isolated from the tissue of Radix Salvia miltiorrhiza Bge,which were composed of 62 endophytic fungis and 7endophytic actinomycetes.They were identified according to morphology and 16 srDNA.The antagonistic activities of the endophytes and some of their secondary metabolites against pathogens were also carried out.The results showed that：（1）Fusarium moniliforme Sheld.and Alternariasp were the dominant genera among endophytes fromSalvia miltiorrhiza;（2）44strains presented antagonistic to pathogenic bacterium or fungi,among of which,50% show antifungal activity on Escherichia coli;Some endophytes had the strongest bacteriostatic activities on Staphylococcus aureus and Candida albicans,the biggest diameter of antagonism could be 25mm;（3）The secondary metabolites of strain A232 presented strong and broad-spectrum and antagonistic activity to C.albicans and Valsa mali.Based on morphology and 16 SrDNA sequence,strain A232 was identified as Streptomyces luteoverticillatus.

关 键 词：内生菌 丹参 分离 拮抗性 

分 类 号：Q93-331[生物学—微生物学] Q939.92