头花蓼对幽门螺杆菌尿素酶活性及基因表达的影响  

Effect of Polygonum capitatum on Urease Activity and Gene Expression of Helicobacter pylori

作　　者：张岚 莫非[2] 张姝[2] 孙朝琴[2] 张婉颖 袁昌增 陆景润 ZHANG Lan MO Fei ZHANG Shu SUN Zhaoqin ZHANG Wanying YUAN Changzeng LU Jingrun(Department of Laboratory Science, People＇s Hospital of Tongren, Tongren 554300, Guihou, China School of Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou, China)

机构地区：[1]铜仁市人民医院检验科,贵州铜仁554300 [2]贵州医科大学医学检验学院,贵州贵阳550004

出　　处：《贵州医科大学学报》2016年第10期1204-1207,共4页Journal of Guizhou Medical University

基　　金：高等学校特色专业建设点[(2010)15];贵州省科技计划课题[黔科合J字(2014)2027号];贵阳市科技计划项目[筑科合同(20141001)号];2014年地方高校国家级大学生创新创业训练计划项目(201410660014);2014年贵州大学生创新创业训练计划项目(201410660014)

摘　　要：目的:观察苗药头花蓼对幽门螺杆菌(H.pylori)尿素酶活性及尿素酶编码基因ure A、ure B表达水平的影响,探讨头花蓼抑制H.pylori的可能机制。方法:H.pylori分为对照组、1/2最低抑菌浓度(MIC)、1/4MIC、1/8MIC头花蓼处理组,分别于不含药的10%脱纤维羊血哥伦比亚血琼脂平板及含2、1、0.5 g/L头花蓼的10%脱纤维羊血哥伦比亚血琼脂平板培养3代,尿素酶活性比色法定量检测各组H.pylori尿素酶活性,Real-time PCR检测对照组、1/2MIC头花蓼处理组尿素酶基因ure A、ure B的mRNA水平。结果:头花蓼作用后,处理组H.pylori尿素酶活性均显著低于对照组,差异具有统计学意义(P<0.05),且头花蓼的浓度越大,H.pylori尿素酶活性受抑制的程度也越大;1/2MIC头花蓼处理后,H.pylori尿素酶编码基因ure A、ure B mRNA水平与对照组比较均显著下调,差异具有统计学意义(P<0.05)。结论:苗药头花蓼可通过抑制H.Pylori尿素酶活性及下调尿素酶基因ure A、ure B的表达水平抑制H.Pylori尿素酶的合成,从而降低H.Pylori在胃酸环境中的生存能力。Objective： To observe the effect of Polygonum capitatum on Helicoba-cter pylori（ H. pylori） urease activity and expression levels of urease gene ure A,ure B,and to discuss the possible mechanisms. Methods： H. pylori was divided into control group,treatment group by Polygonum capitatum with 1 /2 MIC,1 /4 MIC,1 /8 MIC,cultivated after three generations with defibrinated sheep blood Columbia blood agar base and same blood agar base contained 2,1,0. 5 g / L Polygonum capitatum concentration. Adopting Real-time PCR to detect the mRNA level of ure A,ure B of 1 /2 MIC and control group. Results： After processed by Polygonum capitatum,the H. Pylori urease activity of each treatment group were significantly lower than that in control group,the difference was statistically significant（ P〈 0. 05）. The more concentrated the Polygonum capitatum was,inhibition level of such activity was greater; the mRNA level of urease gene ure A,ure B of 1 /2 MIC Polygonum capitatum was significantly down regulated compared with control group,the difference was statistically significant（ P〈 0. 05）. Conclusion： The Polygonum capitatum can decrease the H. pylori viability in acid environment by inhibiting the urease activity of H. pylori and downregulating the urease gene encoding ure A and ure B to inhibit urease synthesizing.

关 键 词：苗药 头花蓼 螺杆菌 幽门 尿素酶 基因 

分 类 号：R965[医药卫生—药理学]