结缔组织生长因子在UPJO引发肾积水儿童患者肾组织中的表达  

Expression of Connective Tissue Growth Factor in Kidney Tissue of Children Patients with Hydronephrosis Caused by UPJO

作　　者：曹广信[1] 李俊峰[2] 张广平[3] 张传光[2] 刘永东[2] 韩建强[3] 商希峰[2] 胡强[2] CAO Guangxin LI Junfeng ZHANG Guangping ZHANG Chuanguang LIU Yongdong HAN Jianqiang SHANG Xifeng HU Qiang(Department of Vascular Surgery, Weifang People＇s Hospital , Weifang 261041, Shandong, China Department of Pediatric Surgery, Weifang People＇s Hospital, Weifang 261041, Shandong, China Weifang Medical University, Weifan 261041, Shandong, China)

机构地区：[1]潍坊市人民医院血管外科,山东潍坊261041 [2]潍坊市人民医院小儿外科,山东潍坊261041 [3]潍坊医学院,山东潍坊261041

出　　处：《贵州医科大学学报》2016年第10期1224-1227,1230,共5页Journal of Guizhou Medical University

基　　金：山东省潍坊市科技发展项目(20090203)

摘　　要：目的：研究结缔组织生长因子（CTGF）在肾盂输尿管连接部梗阻（UPJO）引发肾积水儿童患者肾组织中的表达。方法：20例UPJO引发肾积水组患儿的患肾标本作为观察组,8例肾外伤等儿童肾组织作为对照组,分别取肾实质、肾盂、肾盂输尿管连接处（观察组取狭窄段）、远端输尿管（观察组取靠近狭窄段的1~2 cm）组织标本,应用HE染色观察其形态学特征,免疫组织化学染色法检测其CTGF的表达和分布差性。结果：HE染色可见对照组肾脏各部位无明显病理改变;观察组肾实质见肾小球数目和面积减少、肾小管间质中纤维化明显、呈灶状分布,肾盂及肾盂输尿管连接处的移行上皮细胞被挤压变形;CTGF主要在肾小管上皮细胞胞、肾盂上皮细胞、输尿管上皮细胞及平滑肌细胞胞浆中表达;与对照组比较,除远端输尿管外,观察组肾实质、肾盂、肾盂输尿管连接处的CTGF阳性颗粒面密度值和光密度值均高于对照组,观察组肾实质、肾盂、肾盂输尿管连接处的CTGF阳性表达率和表达强度均高于对照组（P〈0.05）。结论：儿童UPJO引起的肾积水患肾组织中有不同程度的纤维化改变,CTGF在患肾的高表达可能是其发生纤维化的机制之一。Objective： To study expression of connective tissue growth factor（ CTGF） in kidney tissue of children patients with hydronephrosis caused by UPJO. Method： 20 cases kidney specimen of hydronephrosis caused by UPJO in children were enrolled as observation group,and 8 cases of renal trauma tissue of children as control group. The tissue specimen of renal parenchyma,renal pelvis,junction of renal pelvis and ureter（ narrow section of observation group）,and distal ureter were taken respectively. HE staining was adopted to observe their morphological characteristics and immunohistochemical staining was used to detect the expression and distribution difference of CTGF protein. Results： HE staining showed that control group had no significant pathological changes in different parts of the kidney. In the observation group,the number and area of glomeruli of kidney was decreased,the fibrosis was obvious in the renal tubular interstitial,and the transitional epithelial cells in the renal pelvis and the junction renal pelvis and ureter were extruded and deformed. CTGF is mainly expressed in renal tubular epithelial cells,renal pelvis epithelial cells,ureteral epithelial cells and smooth muscle cells. Compared with control group,in addition to the distal ureter,the observation group was higher in the CTGF positive granular surface density and light density of the renal parenchyma,pelvis and renal pelvis. The positive expression rate and the expression intensity of CTGF in the observation group were higher than those in the control group（ P〈 0. 05）. Conclusions： The renal tissue in children patients with hydronephrosis caused by UPJO shows different degrees of fibrosis change,and high expression of CTGF in kidney may be one of the mechanisms of the occurrence of fibration.

关 键 词：结缔组织生长因子 免疫组织化学 肾盂积水 先天性 肾盂输尿管连接部梗阻 图像处理 计算机辅助 

分 类 号：R726[医药卫生—儿科]