Aβ寡聚体对海马神经细胞突触形态及突触蛋白Ng表达的影响  

Neurotoxicity in synapse of primary cultured hippocampal neurons induced by Aβ25-35 oligomers

作　　者：尉荣翠[1] 房迎华[1] 赵秀琴[1] 张道春[1] 黄昕艳[1] 

机构地区：[1]佳木斯大学第二附属医院,黑龙江佳木斯154002

出　　处：《黑龙江医药科学》2016年第5期50-52,54,共4页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学科研项目;编号:s2013-052

摘　　要：目的:研究Aβ25-35寡聚体对原代培养海马神经细胞突触形态及突触蛋白Ng表达的影响。方法:5、10、20umol/L Aβ25一35寡聚体作用于原代培养的海马神经元1h,以免疫细胞化学法检测Ng的表达。结果:与对照组相比:10、20μmol/L Aβ25-35寡聚体致Ng表达减少,差异有显著性(P<0.05,P<0.01),以10μmol/L Aβ25-35组最显著(P<0.01)。结论:Aβ25-35寡聚体对突触有损伤作用与作用浓度有关。Objective： To investigate neurotoxicity in synapses of primary cultured hippocampal neurons induced by Aβ25-35 oligomers. Methods： The hippocampal neurons were incubated by Aβ oligomers with different concentration for 1 hour. Ng was used as index of the measure synaptic damage. The expression of PSD-95 was detected by using immunohistochemical SABC method. Result： Compared with the normal group,the expression of Ng decreased in 1μmol/L Aβ group,5μmol/L Aβ group（ P〈0. 05,P〈0. 01）. The latter is more significant（ P〈0. 01） with statistical significance. Conclusion： Neurotoxicity in Synapse induced by Aβ25-35 oligomers is associated with concentration in a dose-dependent manner.

关 键 词：Aβ25-35寡聚体 原代培养 NG 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]