纳米微囊介导的碱性成纤维细胞生长因子联合低氧诱导因子-1提高随意型皮瓣缺氧耐受的实验研究  

An experimental study on nanoparticle mediatedapplication with b FGF and HIF-1 to increase hypoxia toleranceof random skin flap

作　　者：王之学[1] 肖继州[1] 赵振霞[1] 于强[1] 

机构地区：[1]聊城市第二人民医院烧伤科,山东聊城252600

出　　处：《安徽医药》2016年第9期1645-1648,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨以纳米微囊为载介导的碱性成纤维细胞生长因子(b FGF)联合低氧诱导因子-1(HIF-1)对大鼠随意性皮瓣缺氧耐受的作用。方法 SD大鼠40只,背部设计6 cm×2 cm的随意性皮瓣。采用随机方法分为4组:A组为联合应用含b FGF和HIF-1的观察组;B组为单纯应用b FGF的基因对照组1;C组为单纯应用HIF-1的基因对照组2;D组为应用生理盐水的空白对照组。采用皮瓣下注射的方式,术后测量皮瓣成活面积,病理切片HE染色,皮瓣组织的b FGF和HIF-1免疫组化检测及皮瓣下毛细血管的数目密度统计。结果纳米微囊介导的观察组与其他对照组相比,皮瓣成活率更高,目的蛋白表达和皮瓣新生血管密度计数与对照组差异有统计学意义(P<0.05)。结论以纳米微囊为载体,联合应用b FGF和HIF-1的基因治疗能促进皮瓣血管的增生,显著提高大鼠随意性皮瓣的缺氧耐受,增加其成活率。Objective To investigate the impact of basic fibroblast growth factor（bFGF）and hypoxia-induced factor -1（HIF-1）media-ted by nanoparticleon hypoxia tolerance of random skinflap in rats.Methods We designedone random skin flap of 6cm ×2cm on the back of SD rat（n =40）.Then rats were randomized into four groups：group A as the experimental group with application of bFGF and HIF-1;group Bas the first control group with soleapplication ofbFGF;group C as the second control group with sole application of HIF-1 and group D as blank control group with application of normal saline only.Results The exprimental group which was mediated bynanoparticle,compared with the other control groups,achieved higher survival rate of skin flap.Conclusions Gene therapy with bF-GF and HIF-1 mediated by nanoparticle can promote the neovascularization in skinflap,enhance the hypoxia tolerance and increase the survival rate of random skin flap of rats.

关 键 词：外科皮瓣 纳米囊 成纤维细胞生长因子 芳香烃受体核转位子 新生血管化 生理性 大鼠 

分 类 号：R622[医药卫生—整形外科]