伪狂犬病病毒感染大鼠肛门括约肌神经传导的研究  

Nerve conduction study of pseudorabies virus infection in anal sphincter of rats

作　　者：樊毅[1] 刘洪亮[1] 李萍[1] 黄剑波[1] 杨凡[1] 姜子义[1] 赵军[1] 许思遥 邓益超 殷玥[1] 毛汐语 吕雯婷[1] 朱玲[1,2] 徐志文[1,2] 

机构地区：[1]四川农业大学动物生物技术中心,四川成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《中国兽医科学》2016年第10期1294-1299,共6页Chinese Veterinary Science

基　　金：国家“十二五”科技支撑计划项目(2015BAD12B04);四川省科技支撑计划项目(2014NZ0043);长江学者与创新团队发展计划项目(IRT13083)

摘　　要：伪狂犬病病毒(Pseudorabies virus,PRV)能感染突触连接的神经元,拥有跨神经元传导的功能,被广泛应用于神经示踪研究,当作神经通路的活追踪剂。通过免疫组织化学示踪PRV感染大鼠肛门括约肌的神经传导,用PRV g E/g I/US9三基因缺失株SA215-T人工感染大鼠,将PRV SA215-T导入大鼠肛门括约肌神经细胞内。利用减毒PRV逆神经传导的特性,示踪肛门括约肌神经节细胞、脊神经、脊髓排便中枢、脑干中枢,最后到大脑皮质层这一整个排便反射神经通路。结果显示,随着感染时间的推移,PRV逆向传导经大鼠肛门括约肌、脊神经、脑干中枢、最终到达大脑。这为进一步探索先天性肛门直肠畸形的发病机制奠定了基础。Pseudorabies virus can infect the synaptic connections of neurons. Pseudorabies virus has the function of across the nerve conduction,is widely used in neural tracer study,like a living tracer neural pathways.A gene deletion virus PRV SA215-T was artificially infected rats,and established the artificial infection animal model of rats.PRV SA215-T was injected into the anal sphincter neural cells in rats.We used attenuated PRV inverse nerve conduction characteristics of the tracer anal sphincter ganglion cells,the spinal nerve,spinal cord central brainstem centers,finally to the brain cortex this entire defecation reflex pathway.Immunohistochemical results showed that PRV SA215-T reversed conduction through the anal sphincter in rats,the spinal nerve,brain stem central,eventually reach to the brain as the infection time.This study used immunohistochemical tracer PRV infected anal sphincter for nerve conduction,in order to further explore the pathogenesis of congenital anorectal malformation.

关 键 词：伪狂犬病病毒 免疫组织化学 神经传导 

分 类 号：S852.659.1[农业科学—基础兽医学]