兔脑炎原虫引起脑星形胶质细胞增殖和胶质纤维酸性蛋白的表达  

Proliferation of cerebral astrocytes and expression of glial fibrillary acidic protein induced by Encephalitozoon cuniculi

作　　者：李瑞珍[1] 银梅[1] 唐海蓉[1] 潘耀谦[1] 

机构地区：[1]河南科技学院动物科技学院,河南新乡453003

出　　处：《中国兽医科学》2016年第10期1308-1314,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31372407)

摘　　要：为了研究兔脑炎原虫引起脑组织星形胶质细胞增生的特点和胶质纤维酸性蛋白(GFAP)表达的丰度,经特殊染色、免疫组织化学染色和免疫印迹技术对30只病兔和10只对照兔的脑组织进行了详细的研究。结果显示,病兔的脑组织出现了弥漫性或局灶性非化脓性脑炎病变及不同形态的特异性肉芽肿。用改良的革兰染色可在肉芽肿的上皮样细胞及坏死组织中检出蓝色的兔脑炎原虫;用甲基绿派若宁染色可在上皮样细胞的胞质中检出红色的兔脑炎原虫;用抗-GFAP免疫组织化学染色,见病兔脑组织中有大量星形胶质细胞增生,在脑肉芽肿及血管套周围也有大量反应态星形胶质细胞,使之与正常脑组织隔离,增生的星形胶质细胞的足突在血管周围、脑软膜下和室管膜细胞的基底部形成厚层屏障膜。用免疫印迹技术检测,病兔脑组织中GFAP表达的丰度明显高于对照兔,且与抗-GFAP免疫组织化学染色的结果相一致。研究结果表明,兔脑炎原虫能引起脑组织中星形胶质细胞增生,反应态的星形胶质细胞形成的各种屏障膜可保护脑组织免受更大的损伤。To research the proliferative feature of astrocyte and GFAP expressive abundance in brain tissue by Encephalitozoon cuniculi,the brain tissues of 30 sick rabbits and 10 control rabbits were studied in detail by special staining,immunohistochemistry and Western-blotting.The results in dicated that the diffuse and local nonsuppurative encephalitis different patterns of specific granu lomas were detected in brain tissues of rabbits with encephalitozoonosis.Lots of blue E.cuniculi in epithelioid cell and necrotic tissues of granulomas were checked out by modified Gram stain.A lot of red E.cuniculi in cytoplasm of epithelioid cells were detected by methyl green pyronine stain.Plenty of astrocyte proliferations were in sick rabbit＇s brain tissues and around brain granuloma and vascu lar cuffing by anti-GFAP staining.The foot processes of proliferative astrocytes formed thicker bar rier membranes around blood vessels,under cerebral pia meter and in basilar part of ependymocytes.GFAP abundance in sick rabbit＇s brain tissues was higher than that of in the control rabbits by Westernblotting which was in line with the results of anti-GFAP immunohistochemical staining.The conclusion is that E.cuniculi can induce astrocyte proliferation in brain tissue.All kinds of barrier membrane formed by reactive astrocyte are able to protect brain tissue against more injury.

关 键 词：兔脑炎原虫 星形胶质细胞 胶质纤维酸性蛋白 免疫组织化学 免疫印迹技术 

分 类 号：S852.723[农业科学—基础兽医学]