上海地区人博卡病毒在儿童急性呼吸道感染的研究  

Molecular epidemiological and clinical features of human bocavirus in children with acute respiratory tract infection in Shanghai.

作　　者：宋靖荣[1] 高寒春[2] 林珍[1] 亢娟[1] 叶剑敏[3] 杨子珍 谢志萍[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院儿科,201900 [2]中国疾病预防控制中心病毒病预防控制所,北京100052 [3]上海交通大学医学院附属新华医院,201900 [4]上海普陀区中心医院,201900

出　　处：《中华实验和临床病毒学杂志》2016年第5期452-456,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81100005）

摘　　要：目的了解上海地区人博卡病毒（HBoV）在儿童急性呼吸道感染中的流行情况和临床特点。方法上海地区在2012年1月至2012年12月共收集271例急性呼吸道感染患儿的鼻咽抽吸物，采用巢式聚合酶链反应（Nested PCR）方法检测人博卡病毒NS1基因、并经测序确认，对所获得的基因序列进行同源性和进化分析，博卡病毒阳性样本同时检测鼻病毒、呼吸道合胞病毒、腺病毒等8种呼吸道相关病毒。结果271例标本中共检出HBoV阳性31例，检出率11.4%；21例存在混合感染，全年均有检出，阳性患儿中位年龄17个月（4个月-4岁），诊断包括上呼吸道及下呼吸道感染，临床表现包括发热、阵发性咳嗽、咳痰、腹泻、呕吐、咽充血、湿罗音等，无死亡病例，门诊患者检出率明显高于住院患者；序列分析表明其中29例为HBoV1、2例为HBoV2、与参考株的核苷酸同源性为99%-100%，氨基酸同源性96%-100%。结论HBoV1是上海地区急性呼吸道感染患儿中的重要病原，HBoV2在该地区首次检出，临床症状及诊断无特异性。Objective To investigate the molecular epidemiology and clinical characteristics of human bocavirus in children with acute respiratory tract infection in Shanghai, China. Methods Between January 2012 and December 2012, 271 nasopharyngeal aspiration （NPA） samples were collected from children who had been hospitalized for acute respiratory tract infection at Shanghai, China. HBoV NS1 gene was detected by the nested polymerase chain reaction. In the further, other common respiratory viruses （HRV, RSV, ADV et al） was screened in HBoV positive samples, All PCR positive products were sequenced and phylogenetic analysis. Results The overall frequency of HBoV, infection was 11.4%. 21of 31 HBoV positive sample tested were mix-infection. HBoV was detected from all over year. Children with HBoV varied in age from 4 months to 4-years （median age, 17 months）. The clinical diagnoses of HBoV positive patients included acute upper respiratory tract infection and lower respiratory tract infection. The clinical presentations of HBoV positive children included fever, cough, sputum production, diarrhea, vomiting; pharynx engorgement, crackles, wheezing. There being a statistically significant difference in the detection rates of HBoV between the outpatients and inpatients. The HBoV NS1 gene sequences phylogenetic analysis revealed that 29 HBoV1 NS1 sequences shared 99%-100% nucleotide identity with the human boeavirus strain, whereas the amino acid identity was 96%-100%. The two HBoV2 NSI sequences shared 99% nucleotide sequence identity with HBoV2 strain FJ170282 respectively. Conclusions Human boeavirus was distributed in Shanghai region, HBoV1 was dominant and HBoV2 was detected at the first time. The symptom and clinical diagnoses has no specificity with patients with other common respiratory viruses.

关 键 词：呼吸道感染 人博卡病毒 流行病学 儿童 

分 类 号：R725.6[医药卫生—儿科]