六味地黄方及配伍对去势雌性大鼠动脉粥样硬化模型的作用  

Effect of Liuwei Dihuang Formula and its compatibility on rats with ovariectomy-induced atherosclerosis

作　　者：姚远[1] 吴艳[1] 马志[1] 张亚云[1] 李育[2] 卞慧敏[1,3] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]南京中医药大学基础医学院,南京210023 [3]江苏省中药药效与安全性评价重点实验室,南京210023

出　　处：《中成药》2016年第10期2111-2117,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81173190);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的探讨六味地黄方（熟地黄,山药,泽泻等）及其配伍对更年期动脉粥样硬化模型大鼠的影响。方法用健康雌性大鼠,假手术组切除双侧卵巢下方少量脂肪,其余动物切除双侧卵巢,以高脂饲料喂养并灌胃给予维生素D3复制大鼠更年期动脉粥样硬化模型。30 d后,将总胆固醇〉5-10 mmol/L的大鼠随机分为6组：模型组、苯甲酸雌二醇组、六味地黄方组、熟地/泽泻组、山茱萸/丹皮组、山药/茯苓组,灌胃给药。油红染色检测血管病变程度,放免法检测血中6-酮-前列腺素F1α（6-keto-PGF（1α））、血栓烷素B2（TXB2）、炎性相关因子C反应蛋白（CRP）、肿瘤坏死因子α（TNF-α）、白介素6（IL-6）水平,免疫组化和Western blot法检测大鼠主动脉雌激素膜受体GPR30的表达。结果六味地黄方及其配伍能够不同程度地减轻模型大鼠血管病变,改善血中6-keto-PGF（1α）和TXB2平衡,降低血中炎性因子表达,升高主动脉雌激素膜受体GPR30表达。结论六味地黄方具有治疗更年期动脉粥样硬化的作用,各药对相互配伍协同阻止动脉粥样硬化的形成。AIM To observe the effects of Liuwei Dihuang Formula（ Rehmanniae Radix praeparata, Dioscoreae Rhizoma,Alismatis Rhizoma,etc.） and its compatibility on female rats with ovariectomy-induced atherosclerosis. METHODS The small amount of fat beneath the ovary was removed in the sham group,the other female rats were ovariectomized and fed with a high-fat diet and VD3 to induce rats with atherosclerosis. After 30 days,rats of total cholesterol level more than 5 mmol / L were randomly divided into six groups： model,estradiol benzoate,Liuwei Dihuang Formula,Foxglove ＋ Alisma,Dogwood ＋ Dan,Yam ＋ Poria group,administered orally.Oil red staining was used to detect the degree of vascular lesion. The levels of 6-keto-PGF（1α）,TXB2,CRP,TNF-αand IL-6 in blood were detected by radioimmunoassay. Immunohistochemical and Western blot analyses were used to detect the expression of GPR30 in aorta. RESULTS Liuwei Dihuang Formula and its compatibility reduced vascular disease,improved the balance of 6-keto-PGF（1α）and TXB2,reduced the expression of inflammatory factors in the blood,and increased aortic expression of estrogen receptor GPR30 in model rats. CONCLUSION Liuwei Dihuang Formula has therapeutic effects on menopause atherosclerosis.

关 键 词：六味地黄方 更年期动脉粥样硬化 内皮功能 炎性因子 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]