金雀花中3种黄酮成分的测定及其指纹图谱的建立  

Determination of three flavonoids in Caragana sinica and establishment of its fingerprints

作　　者：武钰[1] 李东雨[1] 姚军[1] 程煜凤[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054

出　　处：《中成药》2016年第10期2204-2208,共5页Chinese Traditional Patent Medicine

基　　金：新疆维吾尔自治区重大科技专项(201130105)

摘　　要：目的通过HPLC法测定金雀花Caragana sinica（Buchoz）Rehd.中槲皮素、山柰素、异鼠李素的含有量,并建立其指纹图谱。方法该植物75%乙醇提取液的分析采用Waters C18色谱柱（150 mm×4.6 mm,5μm）;流动相为甲醇-0.4%磷酸;体积流量1.0 m L/min;柱温30℃;检测波长360 nm。再建立10批样品的指纹图谱。结果槲皮素、山柰素、异鼠李素分别在0.01-0.1、0.002 5-0.025、0.002 5-0.025 mg/m L范围内线性关系良好,平均回收率分别为95.5%、92.3%、95.6%,RSD分别为0.62%、0.89%、0.89%。指纹图谱中有12个共有峰（9号峰为槲皮素,11号峰为山柰素,12号峰为异鼠李素）,相似度均大于0.990。结论提取金雀花时未进行石油醚脱脂可使色谱峰分离度良好,回收率较高。AIM To determine the contents of quercetin,kaempferol and isorhamnetin in Caragana sinica（ Buchoz） Rehd. by HPLC and to establish its fingerprints. METHODS The analysis of 75% ethanol extract of this plant was performed on a 30 ℃ thermostatic Waters C18column（ 150 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of methanol-0. 4% phosphoric acid at a flow rate of 1. 0 m L / min,and detected wavelength was set at 360 nm. Then the fingerprints of ten batches of samples were established. RESULTS Quercetin,kaempferol and isorhamnetin showed good linear relationships within the ranges of 0. 01- 0. 1 mg / m L,0. 002 5-0. 025 mg / m L and 0. 002 5- 0. 025 mg / m L,whose average recoveries were 95. 5%,92. 3% and 95. 6% with the RSDs of 0. 62%,0. 89% and 0. 89%,respectively. There were twelve common peaks in the frigerprints（ Peak 9was quercetin,Peak 11 was kaempferol,and Peak 12 was isorhamnetin）,whose similarities were all more than0. 990. CONCLUSION The extraction of C. sinica without petroleum degrease can make chromatographic peaks＇ separation better and recovery rate higher.

关 键 词：金雀花 槲皮素 山柰素 异鼠李素 指纹图谱 HPLC 

分 类 号：R284.1[医药卫生—中药学]