不同膜材料精制中药复方骨痹颗粒的比较  

Comparing study on different membrane materials to refined Chinese herbal compound of Gubi granules

作　　者：杨磊[1,2] 宗杰[1,2] 朱华旭[1,2] 郭立玮[1,2] 龙观洪 李博[1,2] 潘林梅[1,2] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,南京210023 [2]南京中医药大学江苏省植物药深加工工程研究中心,南京210023

出　　处：《膜科学与技术》2016年第5期116-123,共8页Membrane Science and Technology

基　　金：国家自然科学基金项目(81072749;81303230;30873449;30873450);江苏省科技厅自然科学基金项目(BK2012855);江苏省科技厅产学研联合创新资金--前瞻性研究项目(BY 2012036);江苏省六大人才高峰项目(2014-YY-014)

摘　　要：以中药复方骨痹颗粒为模型药物,选择川续断皂苷Ⅵ、柚皮苷、总黄酮和总皂苷为复方水提液需保留的发挥药效作用的主要指标性成分(药效成分);选择淀粉、蛋白质、果胶和鞣质为复方水提液需主要去除的无效高分子指标性成分;选择中药复方精制后所得固含物(固含率)为精制效果指标,综合研究不同膜材料对中药复方骨痹颗粒上述各类成分的影响.采用高效液相色谱法(HPLC)和紫外分光光度法检测骨痹颗粒水提液中上述各类成分的截留率和保留率,评价不同膜材料对复方水提液的精制效果.结果表明,与聚醚砜(PES)平板膜和PES中空纤维膜相比,0.2μm陶瓷膜对骨痹颗粒水提液中发挥药效作用的主要指标性成分的保留率较高,对高分子指标性成分(蛋白质除外)的截留率较高,且对固含率控制效果最好.将0.2μm陶瓷膜精制工艺与中药复方水提取液传统的乙醇沉淀除杂工艺相比较,结果表明,0.2μm陶瓷膜精制工艺与传统醇沉工艺相比,前者对中药复方的精制效果优于后者.The refining effects of different membrane materials on various components of Chinese herbal compound were studied. GuBi granules, a kind of traditional Chinese prescription which consist of eight kinds of herbs, were chosen as a model, and the yield of micro-molecules such as active ingredients, invalid polymer component and solids transmittance that can be measured from the refined process of different membrances were determined to evaluate the refining efficiency. Meanwhile, high performance liquid chromatography and ultraviolet spectrophotometry were used. The results showed that compared with poly ether sulphone（PES） flat membrane and PES hollow fiber membranes, 0. 2μm ceramic membranes possess better transmission rate for micro-molecules and high retention rate for macromolecules such as starch, pectin, tannin etc. and had a best control to solid content of Chinese medicine. Further experiment was done for comparing 0. 2μm ceramic membrane refining technology with water extract ethanol precipitation process, and the results showed the former had a better control of solid content in Chinese medicine.

关 键 词：中药复方水提取液 骨痹颗粒 膜材料 传统乙醇沉淀工艺 陶瓷膜精制工艺 

分 类 号：TQ461[化学工程—制药化工]