新疆吐鲁番地区三种甜瓜病毒病的发生与分子鉴定  

Occurrence and Molecular Identification of Three Kinds of Melon Virus Diseases in Turpan Area,Xinjiang

作　　者：韩盛[1] 韩成贵[2] 玉山江.麦麦提 杨军 杨渡[1] 

机构地区：[1]新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐830091 [2]国家农业生物技术重点实验室/中国农业大学农学与生物技术学院,北京100193 [3]新疆葡萄瓜果开发研究中心,新疆鄯善838201

出　　处：《新疆农业科学》2016年第10期1829-1842,共14页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科研机构创新发展专项资金项目"新疆甜瓜主要病害监测与系统防控技术研究"(2014006);新疆农业科学院优秀青年科技人才基金项目"新疆甜瓜Polerovirus属与Potyvirus属病毒鉴定与分子变异研究"(xjnky-2012-038);农业生物技术国家重点实验室开放研究课题项目"新疆瓜类作物Polerovirus属与Potyvirus属病毒鉴定与分子变异研究"(2012SKLAB06-3)~~

摘　　要：【目的】明确吐鲁番地区西瓜花叶病毒(WMV)、小西葫芦黄化花叶病毒(ZYMV)、南瓜蚜传黄化病毒(CABYV)的发生情况及三种病毒不同来源分离物间的遗传差异。【方法】采用分子检测方法,确定吐鲁番地区甜瓜CABYV、WMV、ZYMV的侵染比例,应用序列比对及构建系统发育树分析三种病毒,吐鲁番分离物与国内外其他分离物间遗传差异。【结果】吐鲁番地区40份病毒病样中WMV、ZYMV、CABYV分子检测阳性比例分别达87.5%、50%、30%。ZYMV吐鲁番分离物等部分国内外分离物NIB与CP蛋白之间存在蛋白切割位点Q/S,WMV、ZYMV吐鲁番分离物等部分国内外分离物DAG基序的氨基酸序列均为DAG。依据系统发育树分析将三种病毒吐鲁番分离物分别划分至不同的亚组。【结论】WMV、ZYMV为吐鲁番地区甜瓜病毒病的优势毒原,CABYV为吐鲁番地区甜瓜病毒病的主要病毒之一。CABYV、WMV吐鲁番分离物与国内部分省份分离物亲缘关系更近,ZYMV吐鲁番分离物XJturufan2与东欧、中东国家的分离物亲缘关系更近,ZYMV吐鲁番分离物XJturufan与国内部分省份分离物及美国、西班牙、日本等国分离物亲缘关系更近。【Objective】To determine the occurrence situation of WMV,ZYMV,CABYV in Turpan area,and find out genetic differences between these virus isolates from different place.【Method】The Infection proportion of CABYV,WMV,ZYMV that infected melons in Turpan area were determined by the method for molecular detection. The genetic differences between the three kinds of viruses from Turpan and these isolates from different domestic and overseas place was analyzed by the method for sequence alignment and construction of phylogenetic trees.【Result】The positive rates of WMV,ZYMV and CABYV in 40 samples of virus disease in Turpan area were 87. 5%,50% and 30%,respectively. There was a protein cleavage site Q/S between NIB protein 3 ＇terminal and CP protein 5＇ ends of ZYMV isolates that were isolated from Turpan and other domestic and foreign places. The acid sequences of aphid transmission characteristic domains DAG motif of WMV isolates isolated from Turpan and other domestic and foreign places are DAG separation. The three viruses were divided into different subgroups according to phylogenetic tree.【Conclusion】WMV,ZYMV were the dominant viruses that infected muskmelon in Turpan area,CABYV has become one of the major virus causing muskmelon virus disease in Turpan area. CABYV,WMV Turpan isolates were closer to the isolates from China＇s domestic provinces,the ZYMV Turpan isolate XJturufan2 is closer to the isolates from Eastern Europe and the Middle East countries,But ZYMV Turpan isolates XJturufan are closer to the isolates from Zhejiang,Henan,Liaocheng,Taiwan in China and other isolates from The United States,Spain and Japan.

关 键 词：西瓜花叶病毒 小西葫芦黄化花叶病毒 南瓜蚜传黄化病毒 系统发育树 DAG基序 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]