新疆塔城地区野生阿魏菇菌株的遗传多样性分析  

Genetic Diversity Analysis of Wild P. eryngii Strains from Tacheng,Xinjiang

作　　者：贾培松[1] 努尔孜亚.亚力买买提 罗影[1] 郝敬吉吉 贾文捷[1] 杨建终 管建华 魏鹏[1] 

机构地区：[1]新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐830091 [2]新疆第九师102团农业技术服务中心,新疆塔城834704 [3]青河县农业技术推广中心,新疆青河836200

出　　处：《新疆农业科学》2016年第10期1900-1906,共7页Xinjiang Agricultural Sciences

基　　金：现代农业产业技术体系(CARS24);自治区公益性科研院所基本科研业务经费资助项目(KY2014035);新疆农业科学院院长基金项目(xjnky-2012-y04)~~

摘　　要：【目的】研究新疆塔城地区野生阿魏菇菌株的遗传多样性和遗传分化特征,为阿魏菇驯化选育提供基础材料和科学依据。【方法】利用生物学和ISSR标记技术对23株阿魏菇菌株进行遗传多样性分析。【结果】在阿魏菇菌丝培养特征方面,各菌株除在菌丝颜色上无明显差异外,在菌落形态、菌丝长势等7项特征方面均有明显差异;ISSR标记共扩增出60条DNA条带,多态性条带57条,多态比率为95.00%,供试菌株两两间的相异系数从0.07~0.68,D=0.31时,可将23个供试菌株分为13个类群。【结论】新疆塔城地区野生阿魏菇野生阿魏菇菌株已开始发生遗传分化,并具有较丰富的遗传多样性。【Objective】The genetic diversity of wild P. eryngii strains collected from Tacheng,Xinjiang was estimated,and the purpose was to provide basic materials and scientific basis for the further breeding of ferula mushroom.【Method】Biological characteristics and ISSR markers were used to analyze the genetic diversity of 23 P. eryngii strains.【Result】It was shown that except no obvious difference in color,significant differences of cultural characteristics were observed among tested samples in colonial morphology,mycelium growth vigor and so on. It was shown that the percentage of polymorphic bands from ISSR markers was95. 00%. Cluster analysis showed that the 23 strains of P. eryngii could be divided into 13 groups at the level of D = 0. 31.【Conclusion】The natural populations of P. eryngii from Tacheng has the occurrence of genetic differentiation with relatively rich genetic diversity.

关 键 词：野生阿魏菇 遗传多样性 培养特征 ISSR 

分 类 号：S646[农业科学—蔬菜学] S188[农业科学—园艺学]