谷子抗除草剂的质体ACCase基因及系统发育分析  

Herbicide Resistance ACCase Gene in Setaria italic and Molecular Phylogeny

作　　者：高爱保[1,2] 郭平毅[1] 

机构地区：[1]山西农业大学,山西太谷030801 [2]晋中学院,山西晋中030600

出　　处：《中国农学通报》2016年第30期61-67,共7页Chinese Agricultural Science Bulletin

基　　金：山西农业大学博士后基金项目"拿捕净对不同谷子影响及抗拿捕净基因筛选"(NO.614144);山西省重点研发计划项目"谷子全程机械化产业技术开发"(No:2015-TN-09)

摘　　要：为进一步培育抗除草剂谷子,掌握除草剂抗性的分子机制,本研究选择抗拿捕净的谷子品系(‘Setaria italic Chum BC 6-1’)和普通敏感谷子品系(‘S.italic Sda11’)ACCase序列进行了比较,并对谷子近缘的14个物种ACCase基因序列分析,计算碱基组成、转换颠换、遗传距离,构建系统进化树。结果表明:2种谷子ACCase酶序列全长2320个氨基酸,仅有11个氨基酸不同。除已报道较多的I-1779-L对拿捕净抗性外,发现另3个位点S-105-N,D-489-Y,W-876-L突变也可能表现出拿捕净抗性。14个ACCase基因序列平均发生转换259对,颠换213对。利用邻接法和最大似然法分别构建系统进化树,显示相似的拓扑结构,奇异虉草、小子虉草先聚合,然后与燕麦和硬直黑麦草聚合。另一支大穗看麦娘、日本看麦娘先聚合,再和罔草聚合。然后这两支聚在一起。第三支上玉米与稗草首先聚在一起,再和谷子聚集,和物种分类一致。仅小麦单独分出,与传统分类物种树不同。本研究发现另外3个位点突变与拿捕净抗性有关,有利于深入理解除草剂抗性机理,为抗除草剂育种和分子系统发育奠定了基础。In order to breed herbicide resistant millets and master the molecular mechanism, the authors chose ACCase sequences from sethoxydim resistant line‘Setaria italic Chum BC 6-1＇and sensitive line‘S. italicSda11＇to compare, and analyzed ACCase gene sequences of the 14 species. The base composition, transitionand transversion, and genetic distance were calculated, and then the phylogenetic tree was constructed. Theresults showed that the lengths of ACCase sequence of the two lines had 2320 amino acids with 11 differentamino acids. Except the reported I-1779-L locus showed a sethoxydim resistance, we found that the other 3loci mutations, S-105-N, D-489-Y and W-876-L, might also show a sethoxydim resistance. The average 259 pairs of transition and 213 pairs of transversion appeared in 14 ACCase gene sequences. The phylogenetictrees were constructed by Neighbor Joining（NJ） method and Maximum Likelihood（ML） method, showing thesame topologies. Phalaris paradoxa and Phalaris minor were first aggregated, subsequently, Avena sativa andLolium rigidum. Another branch, Alopecurus myosuroides and Alopecurus japonicus were first aggregated,subsequently, Beckmannia syzigachne. Then the both were clustered. The third branch, Zea mays wasaggregated, with Echinochloa crus- galli firstly, clustered with Setaria italic consequently. Only Triticumwas separated, different from the traditional classification. This study found that the other 3 loci wererelated to sethoxydim resistance, which was contributed to understand the mechanism of herbicide resistance.It was the basis for molecular phylogenesis and herbicide resistant breeding.

关 键 词：抗除草剂 乙酰-CoA羧化酶 系统发育 谷子 

分 类 号：S515[农业科学—作物学]