云南蔗区甘蔗线条花叶病毒分离物NIa基因形成新簇  

A novel phylogenetic lineage clustered by NIa gene of Sugarcane streak mosaic virus Yunnan isolates

作　　者：贺振[1,2] 李文凤[3] 李世访[2] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]云南省农业科学院甘蔗研究所,云南省甘蔗遗传改良重点实验室,云南开远661699

出　　处：《微生物学报》2016年第11期1802-1810,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31601604);植物病虫害生物学国家重点实验室开放课题(SKLOF201518);扬州大学科技创新培育基金(2015CXJ043)~~

摘　　要：【目的】利用NIa基因,阐明甘蔗线条花叶病毒(Sugarcane streak mosaic virus,SCSMV)的种系发生关系,为预测SCSMV流行变异趋势及科学防控提供理论依据。【方法】从云南蔗区和国家甘蔗种质资源圃采集感病样品,RT-PCR扩增获得SCSMV NIa基因序列后,使用Splits Tree、RDP、Phy ML、Dna SP等软件分析SCSMV中国分离物的系统发生、选择压力及基因流动等特征。【结果】共获得23条SCSMV NIa基因序列。这些序列间未发生重组,云南蔗区的部分序列形成1个新簇,且云南蔗区与国家甘蔗种质资源圃之间的基因交流不显著。此外,选择压力分析表明,NIa基因受很强的负选择压力作用。【结论】与P1、HC-Pro和CP等基因类似,SCSMV在NIa基因上也包含5个簇;SCSMV云南分离物具有较高的遗传多样性和清晰的地理相关性。[Objective] We assessed the phylogenetic relationship of Sugarcane streak mosaic virus（SCSMV）according to NIa sequences, to infer the prevalence and variation of SCSMV and to prevent and control this virus.[Methods] Leaf samples with mosaic symptom were collected from sugarcane-growing areas in Yunnan province and the Chinese national nursery of sugarcane germplasm resources（NNSGR）. NIa sequences of SCSMV were determined by RT-PCR, and analyzed by Splits Tree, RDP, Phy ML and Dna SP softwares, in aspect of phylogenetic,selection, and gene flow. [Results] We obtained 23 NIa sequences; clear recombination site was not found in NIa; a novel cluster formed by SCSMV Yunnan isolates determined here was found; strong purifying selection was found in NIa of SCSMV; and the gene flow of SCSMV subpopulations between sugarcane-growing areas in Yunnan province and the NNSGR was not frequent. [Conclusion] Similar with P1, HC-Pro and CP genes, SCSMV isolates could be divided into five clusters. NIa of SCSMV Yunnan isolates showed high genetic diversity and clear geographical distribution.

关 键 词：甘蔗线条花叶病毒 NIa基因 系统发生 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]