IL-33参与华支睾吸虫感染后小鼠肝脏嗜酸性粒细胞的维持  

The involvement of IL-33 in the eosinophil infiltration of liver infected by clonorchis sinensis

作　　者：于倩[1,2] 沈莉萍 程晓丹 颜超 汤仁仙[1,2] 郑葵阳[1,2] 

机构地区：[1]徐州医科大学病原生物学与免疫学教研室,221004 [2]徐州医科大学感染与免疫实验室,221004

出　　处：《免疫学杂志》2016年第11期958-960,共3页Immunological Journal

基　　金：国家自然科学基金青年科学基金(31302077);2015年度江苏省脑病生物信息重点实验室开放研究课题(1506);2016年度江苏省高校自然科学面上项目(16KJB310016)

摘　　要：目的探讨在华支睾吸虫感染中,肝脏局部高水平的嗜酸性粒细胞浸润现象是否与内源性白细胞介素33(IL-33)有关。方法建立华支睾吸虫感染小鼠模型,利用流式细胞术检测感染小鼠肝脏嗜酸性粒细胞比例变化;利用抗ST2抗体阻断IL-33与ST2的作用,观察对感染后小鼠肝脏局部嗜酸性粒细胞浸润程度的影响。结果华支睾吸虫感染后,肝脏嗜酸性粒细胞比率显著增多。阻断IL-33与ST2的作用,能显著降低感染小鼠肝脏局部嗜酸性粒细胞比例(12.07%±1.58%vs 7.69%±0.68%;P<0.01)。结论华支睾吸虫感染后,IL-33参与肝脏局部高水平嗜酸性粒细胞的维持。The cytokine IL-33,as a novel alarmin,is an important eosinophil activation factor.However,whether IL-33 is essential in the increasing of eosinophil in clonorchis sinensis infection is unknown.Here,we established clonorchis sinensis infection mouse model,administrated the blocking anti-ST2 antibody in infected mice and detectd the change of the degree of eosinophils infiltration in liver.Data showed that the degree of eosinophilia in inflammatory infiltrating increased significantly after infection.But this increasing is partly reversed by the administration of the blocking anti-ST2 antibody.Those data indicate that IL-33 is involved in the eosinophil infiltration of liver infected by clonorchis sinensis.

关 键 词：华支睾吸虫 白细胞介素33 嗜酸性粒细胞 

分 类 号：R532.23[医药卫生—内科学]